#human embryo crispr

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Mitalipov rebuts CRISPR pub critiques: strengthens case, but puzzles remain

Mitalipov rebuts CRISPR pub critiques: strengthens case, but puzzles remain

Remember that Shoukhrat Mitalipov lab paper on the use of CRISPR in human embryos? It’s back in the news.

Ma, et al. 2018 Nature, FIgure 1

One of the biggest stories of 2017 centered on a Nature paper (Ma, et al., see my quick, initial review shortly after it was published here) from Mitalipov’s lab claiming both efficient repair of a disease-causing mutation in human embryos via CRISPR-Cas9 gene…

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Why we shouldn't view the human embryo as a gizmo even in the CRISPR era

Why we shouldn’t view the human embryo as a gizmo even in the CRISPR era

My first job in science was as a lab technician at UCSD School of Medicine and a big part of that job was growing cells called HUVECs or human umbilical vein endothelial cells. We isolated and grew the HUVECs from umbilical cords that we retrieved from the maternity ward of the UCSD hospital, which first entailed getting the placenta and attached umbilical cord as our starting material.

Some…

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Mulling over that Mitalipov human embryo CRISPR pub, still many questions

Mulling over that Mitalipov human embryo CRISPR pub, still many questions

Modified open Wikimedia image to symbolize an 8-cell CRISPR’d human embryo

What really happened at the DNA level in the experiments in that high-profile CRISPR of human embryos paper from a team led by Shoukhrat Mitalipov at OHSU?

Is the team right that they successfully conducted CRISPR of human embryos to correct a mutant gene, as they reported in their Ma, et al. Nature paper?

Or is the Egli,…

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Mitalipov briefly responds to Egli preprint, stands by human embryo CRISPR pub findings

Mitalipov briefly responds to Egli preprint, stands by human embryo CRISPR pub findings

Shoukhrat Mitalipov points to an image of a CRISPR edited human embryo inside an incubator at the Center for Embryonic Cell and Gene Therapy in Portland, Ore. Rob Stein/NPR.org

There has been a wave of intense discussions both in the public domain such as on Twitter and behind the scenes over the new Egli, et al. preprint that challenges the main conclusions of the Ma, et al. Naturepaper from…

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Human embryo CRISPR pub includes plagiarism: the victim's unique account

Human embryo CRISPR pub includes plagiarism: the victim’s unique account

Cut, modify, paste…

It’s kind of a CRISPR mantra for those of us using gene editing in the lab. But it’s supposed to be happening just on DNA, right?

Now it appears that someone on a team of human embryo CRISPR researchers possibly got carried away with the cutting-modifying-pasting mindset to take it beyond DNA to also do so with passages of another researcher’s published work that they…

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Q&A video with Paul Episode 2: stem cell & CRISPR questions answered

Q&A video with Paul Episode 2: stem cell & CRISPR questions answered

This is the second in a series of videos I’m doing where I answer questions about the stem cell field. You can see the Episode 1 video here.

Today I tackle more questions on the stem cell field. If you have more stem cell questions, let me know and I’ll try to answer them next time.

One reader asked me about stem cells for stroke…

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