ASTM E2180 - Antimicrobial Test for Hydrophobic Materials
ASTM E2180 test is a quantitative method used for determining the antimicrobial effectiveness of the agent(s) incorporated in polymeric or hydrophobic surfaces.
ASTM E2180 Test Procedure
Agar slurry inoculated by test microorganisms is spread onto the test (treated) and control (untreated) sample of hydrophobic material.
After a contact period of 24 hrs, microorganisms from both control and test samples are recovered separately using a neutralizing solution.
Serial dilutions are made and plated on agar medium. These plates are further incubated for 48 hrs under specific conditions optimal for the growth of test organisms.
Bacteria colonies are counted and antimicrobial activity is calculated by comparing the reduction of bacterial colonies in treated samples with bacteria colonies present on untreated samples.