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Where to find effective protein quantification products?
Measuring the concentration of protein sample is important to a lot of experiments, such as western blotting. In two-dimensional gel electrophoresis, too many or too little protein content will both bring serious effect to the result of your experiment. Abbkine offers BCA assay and Bradford assay to help you quantify the protein in your sample, and helps your downstream experiment.
The BCA protein assay is a widely used method for colorimetric detection and quantification of total protein in a solution.
(Picture Source: Labome)
Figure 1. Mechanism of BCA assay.
The Bradford assay is the fastest and easiest way to perform among the protein assays and uses about the same amount of protein as the Lowry assay.
(Picture Source: Labome)
Figure 2. Mechanism of Bradford assay.
Protein quantification kits are common reagents in biology labs, and most of commercial kits have the same principle, with BSA as standard. Below are some comparison data against Abbkine products, you can refer to it and choose properly:
CAT#KTD3001 Protein Quantification Kit (BCA Assay)
FeaturesFigure 3. BCA assay –Standard Curve at 37°C
1.Time saving, much easier and four times faster than the classical Lowry method.
2.Sensitive, with the minimum amount of detection protein reaches 0.5μg.
3.Compatible, unaffected by most ionic and nonionic detergents and can be compatible with up to 5 % SDS, 5 % Triton X-100, 5 % Tween-20, 60, 80 in the sample.
4.Good linearity and excellent uniformity, exhibiting less protein-to-protein variation than dye- binding methods.
CAT#KTD3002 Protein Quantification Kit (Bradford Assay)
Figure 4. Bradford Assay-Standard Curve
Features
1.Time saving, much easier and four times faster than the classical Lowry method.
2.Sensitive, with the minimum amount of detection protein reaches 0.5μg.
3.Compatible, unaffected by most ionic and nonionic detergents and can be compatible with up to 5 % SDS, 5 % Triton X-100, 5 % Tween-20, 60, 80 in the sample.
4.Good linearity and excellent uniformity, exhibiting less protein-to-protein variation than dye- binding methods.
Tips:
If the sample contains high concentration of detergent (such as SDS, Triton X-100, Tween 20), it is better to choose CAT#KTD3001 Protein Quantification Kit (BCA Assay).
If the sample contains high concentration of reductant (such as DTT, TCEP, BME), it is better to choose CAT#KTD3002 Protein Quantification Kit (Bradford Assay).
Compared with the same kit from other brands, Abbkine could offer diverse sizes of items for customers, including the small package. You can refer to detailed information in below chart:
CAT# Product Name Product Size&Price KTD3001 Protein Quantification Kit (BCA Assay) $45/500T,$100/2000T,$180/5000T KTD3002 Protein Quantification Kit (Bradford Assay) $30/500T,$70/2000T,$120/5000T
With good consistency and high cost performance, we believe Abbkine protein quantification kits will be popular among customers all over the world.
About Abbkine Scientific Co., Ltd.
Abbkine offers services for global scientists in the fields of proteomics and cytology, and is committed to the innovation and development of various scientific reagents related to proteomics and cytology, to promote the development of fields including life science research and drug discovery. Proteomics products cover the preparation of samples (protein extraction, purification, coupling), protein quantification, antibodies and kits for protein detection. Cytology products involve cytokines (cell culture), cell status detection, cell staining, organelle extraction, cell metabolism and cytopathology reagents (kits). Abbkine relies on the product portfolio and unique marketing support as the main market strategy and product innovation mode, to help your research career!
