Jan 6, 2015
Derivation and Culture of Induced Pluripotent Stem Cells on an Artificial Substrate
Derivation and Culture of Induced Pluripotent Stem Cells on an Artificial Substrate
When mouse embryonic stem cells were first derived in 1981 independently by Gail Martin at UCSF and Evans and Kaufman at Cambridge University, the inner cell mass cells from the blastocyst-stage mouse embryos were cultured on a layer of mouse skin cells that had been treated with a drug that prevented them from dividing or with radiation that did the same. These single layers of mouse skin…
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