The current research work was mainly focused on the determination of the composition of gallstones laproscopically removed from the patients in Khairpur Sindh, Pakistan. Analysis of constitutes of gallstone in patients is carried out by FTIR from patients hospitalized in Khairpur Medical College Civil Hospital and from Gambat Institute of Medical Sciences Hospital during 2018 to 2019. A total of 100 (20 males and 80 females) samples of stones laproscopically removed from patients were collected and studied. Cholesterol, pure calcium carbonate, cholesterol + calcium carbonate, cholesterol + bilirubin and calcium bilirubinate stones were found in 65 (65%), 7 (7%), 10 (10%), 12 (12%) and 6 (6%) patients respectively. It is clear from examination of gallstones with Fourier Transform Infrared (FTIR) Spectroscopy that in gallstones the predominant component was cholesterol either only in cholesterol stones or in mixed stones in combination with other components.