Spodoptera frugiperda is one of the most important pests of maize, which can cause significant economic losses, up to 4.8 billion dollars. This study aims to evaluate the bioinsecticidal activity of Bacillus thuringiensis var. kurstaki HD-1 on this caterpillar. For this purpose, two formulations based on Btk HD -1 were tested in vitro against L2 and L3 stage larvae of S. frugiperda at different concentrations for seven days. After one to five days of treatments the local formulation induced reduced mobility, cessation of feeding, color change, desiccation and mortality of larvae. However, the commercial formulation was more effective with the 6.5% concentration. It induced 100% mortality in three days of testing on L2 larvae versus five days for the local formulation and four days on L3 larvae. The LC50 obtained with L2 and L3 were 2.14 and 2.29% for the commercial formulation against 5.3 and 5.5% for the local formulation. This local formulation could be an interesting alternative in an integrated strategy for the control of Spodoptera frugiperda in Côte d’Ivoire in maize crops.