Recently, Cd-TiO2 Nanocomposite has been synthesized by sol gel methods for the study of the antibacterial activity on Gram positive and Gram-negative bacteria by well diffusion method. This prepared Cd-TiO2 Nanocomposite have been characterized by X‐ray diffraction (XRD), Scanning electron microscopy (SEM- EDAX), Ultra Violet Spectroscopy (UV), Fourier Transform Infrared Spectroscopy (FTIR) and Surface Area Analysis (BET). The respective XRD patterns indicate crystalline nature having a rhombohedral structure. The particle size of Cd-TiO2 nanocomposite in 35nm. SEM images confirm the spherical morphology of the sample. The purity of the synthesized Cd-TiO2 Nanocomposite has been confirmed from the energy‐dispersive X‐ray (EDAX) spectra and then C, O, Ti, Cd, Pt element are present in Cd-TiO2 Nanocomposite. The weight percentage of Cd is 5.8%, Ti is 51.03%, C is 5.13% and O is 31.75% in Cd-TiO2 Nanocomposite. BET image shows that the major pore size distribution of Cd-TiO2 nanocomposites is ranged from 2.24nm. The Cd-TiO2 nanocomposite showed that the antibacterial activity when tested against the pathogens only gram-negative bacteria such as Pseodomonous. The zone of minimum inhibition concentration was measured in a range of 20mm in 25μl and 30mm in 100μl.