@ghostly-smoke answered your question “i can’t find a buffer that i need and don’t know the recipe for it...”
does the lab have any common place to put procedures and recipes? a binder or a google drive? if not, try the grad student anyway
as far as i know, there isn’t a central place. i searched through the pile of protocols on our (my grad student’s and my) bench and couldn’t find it
eventually i gave up and left the cells underneath resuspension buffer (i had meant to lyse them today). that’ll probably give me an awful blot because proteolysis, but eventually there wasn’t much i could do ¯\_(ツ)_/¯ i’ll message my grad student now though and i live close enough that i can go back to save the sample if she responds haha