Microbial Genome Editing
With the development of modern molecular biology technology, there are many new methods of microbial breeding with more directional and positive mutation, which greatly promotes the accuracy and efficiency of microbial genome editing.
1. Microbial genome editing based on homologous recombination uses its own Rec system to carry out homologous recombination of exogenous DNA to achieve allelic replacement of target genes, and recombination of genetic information between two homologous strands of DNA.
2. CRISPR/Cas9 method for microbial genome editing (including gene knockout, site-directed mutation, site-directed insertion of foreign sequences, etc.). Compared with traditional gene knockout methods, this method is more convenient for subsequent experimental research.