Nov 17, 2023
In E. coli prokaryotic expression vector pET32a, does the TrxA tag lead to non-specific binding in WB and ELISA assays?
In E. coli prokaryotic expression vector pET32a, does the TrxA tag lead to non-specific binding in WB and ELISA assays? A TrxA tag is often used to make sure that recombinant proteins fold and dissolve correctly in E. coli expression systems and other studies that use protein expression. Because this tag is not found naturally in human cells, Western blotting (WB) and enzyme-linked immunosorbent…
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