#primary human cells

[kosheeka]

Immortal Cell Line

Although cell lines are easy to work with, the physiological relevance of those studies might not be high. They do not show resemblance to human body metabolism and physiology, or even morphology. The immortalization and serial passaging cause several variations in genotype and phenotype of these cells. Due to the lack of morphological or functional features, cell lines might not be able to induce relevant biomarkers. Therefore, it is always better to validate cell lines before use to make sure they are not misidentified or contaminated.

For more info on advantages and disadvantages of primary cells in culture, read here.