Monash University medical research
A cure for congenital sight impairment caused by lens damage is closer following research by scientists at Monash University.
Associate Professor Tiziano Barberi and Dr Isabella Mengarelli from the Australian Regenerative Medicine Institute (ARMI) at Monash University are closer to growing parts of the human eye in the lab. They have, for the first time, derived and purified lens epithelium—the embryonic tissue from which the lens of the eye develops. The purity of the cells paves the way for future applications in regenerative medicine.
Further, the researchers caused these precursor cells to differentiate further into lens cells, providing a platform to test new drugs on human tissue in the lab.
Pluripotent stem cells have the ability to become any cell in the human body including, skin, blood and brain matter. Once the stem cells have begun to differentiate, the challenge for researchers is to control the process and produce only the desired, specific cells.
Using a technology known as fluorescence activated cell sorting (FACS), Associate Professor Barberi and his team were able to identify the precise combination of protein markers expressed in the lens epithelium that enabled them to isolate those cells from the rest of the cultures. Most markers are common to more than one type of cell, making it challenging to determine the exact mix of markers unique to the desired cells.
Monash University Associate Professor Barberi said this breakthrough would eventually help cure visual impairment caused by congenital cataracts or severe damage to the lens from injury, through lens transplants.
“The lens has to some extent, the ability to heal well following surgical intervention; however, with congenital cataracts, the fault is wired into the DNA, so the lens will re-grow with the original impairment. This problem is particularly prevalent in developing countries,” he said.
Combined with advances in producing pluripotent stem cells from fully differentiated adult cells, the research will also progress treatments for eye diseases.
“In the future, we will be able to take adult skin cells, for example, and turn back the clock to produce stem cells. From there, using processes like we have developed for lens epithelium, we will be able to produce diseased cells—an invaluable asset for medical research,” Associate Professor Barberi said.
The researchers will now focus on creating a lens more closely resembling a human eye in the lab.
“The lens cells that we created in the Petri dish are organized differently to those in a human eye. The next challenge is mimicking nature more perfectly,” Associate Professor Barberi said.
About the Australian Regenerative Medicine Institute
The Australian Regenerative Medicine Institute is a $153-million centre that officially opened in April 2009. It is based at the Monash University Clayton campus.
Established through a joint venture between Monash University and the Victorian Government, ARMI builds on the university’s existing strengths in biomedical research and supports the critical infrastructure required to deliver the next generation of discoveries in regenerative medicine.
At full capacity ARMI will be one of the world’s largest regenerative medicine and stem cell research centres. Its scientists focus on unraveling the basic mechanisms of the regenerative process, aiming to eventually enable doctors to prevent, halt and reverse damage to vital organs due to disease, injury or genetic conditions.
This work will form the basis of treatments for conditions such as neurodegenerative disorders, diabetes, arthritis, musculo-skeletal and cardiovascular diseases.
Monash University Medical Science and Research Programs
Researchers bring real and positive change to people all over the world. Monash University encourages students to become a part of that global change.
Monash University offers a large range of research degrees, giving you the opportunity to research whatever you are most passionate about. These include degrees that allow you to mix coursework and professional practice and conduct research across disciplines.
Doctor of Philosophy (PhD)
Master of Philosophy (MPhil)
The Faculty of Medicine, Nursing and Health Sciences at Monash University offers a master’s qualifying course and a range of research degrees (doctorates and master’s courses) that may be undertaken in any of the faculty’s schools. The faculty’s research programs are world renowned, especially in the areas of reproductive biology, embryonic stem cells and IVF as well as other research areas which one day promise to significantly contribute to the defence against degenerative conditions. Other research areas include investigations at the molecular and cellular levels, clinical research, public health interventions and the evaluation of health services. The Faculty of Medicine, Nursing and Health Sciences schools include
Eastern Health Clinical School
School for Indigenous Health
School of Psychology and Psychiatry
School of Public Health and Preventive Medicine
OzTREKK is the Canadian Application and Information Centre for Canadian students applying to or inquiring about study abroad, undergraduate and graduate/professional and research programs in Australia. Contact OzTREKK for more information about postgraduate medical science degrees and research degrees in Australia.