Microscopic Analysis of Sharp Force Trauma in Bone and Cartilage: A Validation Study
Christian Crowder Ph.D., Christopher W. Rainwater M.S., Jeannette S. Fridie M.A.
Sharp force trauma research lacks agreement on reported error rates for correctly identifying toolmark characteristics on bone and cartilage. This study provides error rates for determining blade class (serrated, partially serrated, nonserrated) and type of edge bevel (left, right, even). Three analysts examined cuts to a wax medium, cartilage, and bone using two types of microscopes. Additionally, the observers examined impressions taken from the wax medium and the cartilage. Overall, a total of 504 observations were performed. Serrated blades were distinguishable from nonserrated blades due to their patterned striations. Some difficulties were encountered in distinguishing serrated and partially serrated blades; however, when these groups were considered together as one classification type (serrated), classification accuracy improved from 79% to 96%. Classification accuracy for edge bevel was 65%. Error rates were similar when comparing direct observation of the cut marks versus indirect observation (impressions). Additionally, the type of microscope used did not affect error rates.
Serrated blades were generally distinguishable from nonserrated blades due to their distinct, patterned striations. Error rates were low and generally corresponded to the experience level of the observer suggesting additional training could further reduce error. Partially serrated blades can be problematic as the signature left behind on the bone or cartilage is dependent on the portion of the blade impacting the material as well as the overall contact area. Additionally, there may also be a discrepancy as to when a particular blade would be considered partially serrated (i.e., if only a short portion of the blade exhibits partial serration). The authors therefore suggest analysts choose their wording carefully when submitting reports and consider using phrases such as “an impact with a serrated blade or blade portion” instead of “an impact with a serrated blade.” The same is true for the determination of an impact with a nonserrated blade.
The angle of the impact of the blade is the most influential aspect of the distance between striations left by blade teeth. As the blade moves, the angle of the blade will change, and as a result, the distance between the striations will also change; serrated blades, however, will still produce patterned striations despite these changes in movement, whereas nonserrated blades will produce fine striations that are unpatterned, if visible at all. Thus, it is the pattern of the striations that are important, not the distance.
Additionally, the other incorrect classifications are also particularly informative. Figure 3 shows a cut cartilage from one of the two serrated blades that were incorrectly classified as a nonserrated blade impact. With the serrations being so far apart, there is less potential for striations to be left on the material. In addition, the rounded, scalloped appearance of the serrations may not leave the same marks as typically pointed, sharp teeth of serrated blades. Thus, the distance between serrations, the morphology of the serrations, and the amount of tissue impacted will affect the ability to perform the analysis.
Edge bevel was much more easily distinguished in the casting wax than the bone. One possibility for this is the construction of the wax blocks. The cuts on the rectangular wax blocks were made on a flat surface and the resulting kerf may have been more easily observed from the block rather than the rounded, irregular surfaces of the bone samples. Overall, assessment of blade bevel produced poor results. The bevel angle or degrees of the bevel angle may vary depending on the type of knife (e.g., kitchen vs. sporting knife), which could impact edge bevel classification. This study did not evaluate the degree of edge bevel for each knife; therefore, future research should consider this and determine whether degree of edge bevel correlates to classification accuracy.
During the assessment of edge bevel, two other features were noted in relatively low frequencies on the specimens: Exit scraping was occasionally noted on the side of the bone that individual was standing when the cut was made (Fig. 2). This is expressed as a superficial groove that is likely the result of the blade falling out of the kerf and was always noted on the handle side of the cut. Several bones also exhibit kerf margin deformation where a buildup of ragged, deformed bone appears along the kerf margin (Fig. 2). In serrated blades, this feature was noted on the side of the kerf that corresponded to the side of the edge bevel of the blade. In nonserrated blades, kerf margin deformation was noted but was not consistently associated with the side of edge bevel. In most cases, the amount of deformation seen in serrated blades was greater than that seen in nonserrated blades, which may be associated with the size or spacing of the scallops (serrations) in the blade. With more experimental research, these features may assist future researchers attempting to distinguish the side of edge bevel.