Antimicrobial resistance is a global public health concern involving bacteria becoming resistant to antibiotics, making infections harder to treat. This study aimed to isolate and identify bacteria associated with eye infections in humans in Khartoum State. We collected 121 eye swabs from patients at Abdel Fadeel Almaz Hospital between February and May 2017. Of these samples, 85 (70.3%) yielded bacterial isolates. Identified Gram-positive bacteria included Staphylococcus spp and Bacillus spp, while Gram-negative bacteria included Pseudomonas spp. The predominant bacterium was Staphylococcus aureus. Antibiotic sensitivity tests showed that all isolates were sensitive to ciprofloxacin and azithromycin, indicating these drugs as effective treatments for eye infections. The study concluded that Gram-positive bacteria are the most common causative agents of eye infections in humans, and ciprofloxacin and azithromycin are effective treatment options.