Tiny finger-like projections called filopodia drive invasive behavior in a rare subset of lung cancer cells, researchers at Winship Cancer Institute of Emory University have found.
Adam Marcusâ lab has developed innovative techniques for separating âleadersâ and âfollowers,â subpopulations of tumor cells that cooperate during the process of metastasis. The labâs new analysis of what molecular features distinguish leader from follower lung cancer cells focuses on filopodia. The results are published in Science Advances.
âFilopodia are like the fingers of the cell, and help the cell pull its way forward,â Summerbell says. See the supplementary video for a depiction of filopodia and invasive behavior.
Having longer filopodia is linked with a gene called MYO10, which encodes a component of the internal cellular skeleton stabilizing filopodia, Summerbell and Mouw found. MYO10 was the gene that was the most up-regulated and hypomethylated in leader cells, compared with follower cells, and both long filopodia and invasive behavior depend on MYO10 activity.
âIt was known that MYO10 was linked to invasion and metastasis, but this is the first evidence that it is playing this specific role in a rare subset of cells,â Marcus says. âThis could help us look for these rare cells in patient tumors to gauge how potentially invasive they are.â
Leader cells also secrete fibronectin, a sticky extracellular protein, while follower cells do not. The MYO10 protein helps filopodia rearrange fibronectin molecules into fibrils, but it does not appear to interact with fibronectin directly.
âAs the leader cell filopodia pull on the extracellular matrix, they change this matrix from a random mesh into long parallel tracks in front of the cell, paving a road for a group of cells,â Summerbell says.
Filopodia are sometimes described as resembling antennae â or precursors of more stable cellular structures.
âWeâre observing that in leader cells, filopodia are not only sensors of the extracellular environment but also actively participate in reorganizing the extracellular matrix,â Marcus says.
Summerbell and Mouw also studied other changes that distinguish leader cells, such as elevated expression of the Jagged1 gene. Jagged1 encodes a receptor for the Notch pathway, whose activity lies behind activation of MYO10. MYO10 and Jagged/Notch activation may be generalizable to patient samples and other types of cancer.
The paper involved collaboration with Jeanne Kowalski, PhD, now at University of Texas, Austin and Paula Vertino, PhD at University of Rochester.
The research was supported by the National Cancer Institute (R01CA236369, R01CA194027, R21CA201744, U54CA209992), including three student-initiated F31 grants (F31CA210601, F31CA186676, F31CA180511). Additional support came from the Integrated Core Facilities at Emory, Winship Cancer Institute developmental funds and a postdoctoral fellowship from the American Cancer Society.
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