This seating arrangement is such a manipulative move enforced by Reginald, because if it wasnāt, they would all be in number order.Ā
Let me bore you and analyse it.
First and second we have Klaus and Allison, the two who, out of all of them, appear to be theĀ āweakestā of the six, openly sobbing and huddling together. Putting them first is nothing but an emotionally manipulative move on dear old Reggieās part. Thereās no way after watching the both of them cry through the ordeal are the others going to bolt, because that would be abandoning them to deal with it together. This means that they are emotionally invested in seeing this through, even if they desperately donāt want to do it. Luther wonāt leave Allison, and Ben and Five (because, looking at their interactions, I do believe they were at least somewhat close to their brother before vanishing/dying) wonāt leave Klaus.
Diego is next, and importantly, before Luther. This is nothing but Reginald pitting Number Two vs Number One. For Diego, he is proving his worth by not crying, not whimpering, not holding Graceās hand, and in his head setting the bar as high as he can so he can show up his brother when itās Lutherās turn. Maybe Diego canāt be Number One, but heāll beat Luther at this.
So, next we have Luther, who A) Wonāt leave Allison and Klaus upset and the only ones to suffer through this, B) Will rise to Diegoās challenge, because Reginald has drilled into him he is Number One and needs to set a standard, and C) Will lead the way for his other two brothers who are nervously sat next to him with building anxiety. For him this is a test, both from Diego but also Reginald getting Luther to demonstrate his leadership, and he needs to rise to it.
Ben, I think, is mildly a sheep in Reginaldās eyes. Get the flock to move, and he will follow. This is kinda shown in the bank robbing scene, where all his siblings are getting involved, and only with minimal persuasion Ben does too. This is possibly why heās wedged between siblings, rather than at the end where, numerically, he should be. If Ben was last, he would stew in his obvious nerves, waiting and waiting and waiting until it was his turn, which could lead to him running (depending on how close he is to his siblings, but I doubt heād leave) or The Horror reacting to his emotions and emerging. By having Five last, Ben doesnāt have to wait the longest, and can follow in the footsteps of their leader. He can also have Five there to look at while having his done, because everyone else is stood behind the chair, and if he felt as if he was alone, Ben might panic, which, again, could result in The Horror.
Finally, we have Five at the end. While Five definitely wanted to improve and push his powers possibly more than anyone, I donāt think that means Five was completely up for all of Reginaldās ideas and schemes. Out of all of them, Five has the easiest out. If Reginald tried to make Five go first, I think he would have very easily talked back and jumped elsewhere, which would hinder everything else. If Five isnāt having it and was able to back out, Klaus, Allison, and Ben would gang up and refuse as well. Luther probably would have his, and Diego just to show him up, but Reginald would overall lose control of the situation. Putting Five between Luther and Diego would only interrupt the Number One vs Number Two challenge, and putting Allison and Klaus last wouldnāt leave the emotion tie for the others. With the need to keep Ben following along, Five at the end makes the most sense. It also means he has the most emotional investment, having watched all his siblings (minus Vanya) suffer through their tattoos, therefore locking him to the room because he canāt leave them there and opt out. Five can be selfish, but heās not going to abandon them.
In my mind, Reginald set this up, because heās a sadistic prick who wants complete control over his children and knew exactly what he was doing