Journal of Biodiversity and Environmental Sciences

Journal of Biodiversity and Environmental Sciences (JBES) is a fast and peer-reviewed journal and is scheduled to publish 12 issues in a year. It publishes original research papers, short communications and review papers on the main aspects of...

[jbesinnspub]

Mulch and planting method on quantitative traits of cucumber

See on Scoop.it - Education

Water deficiency and broomrape (Orobanche spp.) infestation are important limitations for cucumber (Cucumis sativus L.) production in Iran. Effects of mulch type (clear polyethylene mulch, black polyethylene mulch, hydro flume mulch, and no mulch), and planting method (seeded and transplanted) were tested to determine effects on cucumber, cv. Super Dominus. The interaction between mulch and planting method affected the number of days to flowering, plant length, number of days to harvest, and earliness. Early yield and yield per plant was highest (186.42 and 183.12 g/plant, respectively) when transplanting was with clear and black polyethylene mulch. Black mulch produced the highest total yield, 1671.75 g per plant. Clear mulch was most effective in controlling broomrape. Using polyethylene mulch (black and clear), and transplanting produced the highest early yield and mulch alone produced the highest yield and control of broomrape. Get more articles at: http://www.innspub.net/volume-6-number-1-january-

See on figshare.com

[jbesinnspub]

Dynamics of growth and phytomass allocation in seedlings of Pistacia atlantica desf. versus Pistacia vera L. under salt stress

See on Scoop.it - Education

Salinity represents a major environmental constraint which can severely limit plant establishment in the arid and semi-arid areas in Tunisia. The present work was carried out to study the response of Pistacia vera L. and Pistacia atlantica Desf. seedlings to salt stress. Evaluation of salinity effects on both species was investigated using five increasing NaCl concentrations (0, 20, 40, 60 and 80 mM) during 60 days. The exposure to NaCl at seedlings stages, affects the majority of the studied parameters. Morphological parameters, such as height of shoot, number of green leaves, leaf area and consequently, phytomass allocation were significantly decreased. Additionally, P. vera accelerated leaf senescence. Both species showed a preferential allocation of the resources in favor of the roots for concentrations of NaCl lower than 40 mM. Whereas, only P. atlantica maintained a root growth for higher concentrations. Under salt stress, the specific leaf area decreased in P. atlantica (p<0.001) and translate a mechanism of tolerance to salinity. It appeared that both species tolerate concentrations lower than 40 mM of NaCl. However, for higher concentrations, P. atlantica seedlings are more tolerant. Get more: http://www.innspub.net/volume-6-number-1-january-2015-ijaar/

See on figshare.com

[jbesinnspub]

Characteristics of saline soil and effect of fertilizer application to rice yield

See on Scoop.it - Education

Characteristics of saline soil determine the rice yield along the seaboard. High concentration of dissolved salt decreases growth and rice yield. The study aimed to evaluate physical and chemical characteristics of saline soil in Sidoarjo and Surabaya, East Java, Indonesia. The result analysis of soil exchangeable Na+, K+, Ca++, and Mg++respectively was 0.8-1.94; 0.33-2.73; 16.32-20.4, 1.83-8.88 me.100g-1.The value of soil pH was 7.35- 7.55, EC value of soil was 0.64-1.83dS.m-1, and the content of organic-C was between 1.1-2,4,6 %. The result of soil characterization was then crosschecked with the rice yield in saline soil by weighing dry rice grains per clump. The rice yield was 3-4.1 ton.ha-1 and negatively correlated to the exchangeable values of Na, SAR, bulk density and dust content. It was positively correlated with organic-C, fertilizer, exchangeableof Ca++, Mg++, and K+, as well as soil’s CEC. The ratio value of Ca:Mgnamely2.2-8.2, and K:Mgnamely0.18- 0.21 exceeded the limit of ideal value and caused low production. The rice yield was negatively correlated with the content of exchangeableNa+, values of pH and EC. It achieved more than 4 ton.ha-1when added with 300-450 g.plant-1 of organic materials and 1.0-1.3 g.plant-1 of NPK. Get more: http://www.innspub.net/volume-6-number-1-january-2015-ijaar/

See on figshare.com

[jbesinnspub]

Morpho-agronomic characterization of cucumber germplasm for yield and yield associated traits

See on Scoop.it - Education

In present research work 24 genotypes of cucumber were evaluated to find out their similarities and differences based on numerical traits. The experiment was laid out in Randomized Complete Block Design (RBCD) with two replications. Numerical traits of the genotypes were measured according to the coding criteria specified by European Cooperative Programmed for Plant Genetics Resources (ECPGR) 2008. Data showed great variation for almost all the traits. Maximum germination (67.5%) was observed in genotype Mardan local while minimum germination (17.5%) was recorded in genotype 28295. The genotypes Haripur local and 28293 showed early flowering. Similarly highest yield was observed in USA Poinsett, Dargai local and Mardan local. These genotypes could be chosen for crossing with other genotypes like 28295 and Sialkot selection having low germination rate and low yield to get a better genotype of cucumber with high germination and maximum yield. Correlation analysis represent that yield was positively correlated with fruit length (.523** P≤0.01) and fruit width (.439* P≤0.01). While fruit per plant showed positive significant correlation with vine length. Present findings are applicable in cucumber cultivation in swat and other areas with similar climatic conditions. This will intern help to improve the economic return and revenue generation of the farmers. Get more: http://www.innspub.net/volume-6-number-1-january-2015-ijaar/

See on figshare.com

[jbesinnspub]

Incidence and distribution of cassava mosaic begomoviruses in Côte d’Ivoire

See on Scoop.it - Education

Cassava mosaic disease (CMD) caused by the whitefly- transmitted begomoviruses (family Geminiviridae) is a major threat to production of cassava (Manihot esculenta Crantz) in Côte d’Ivoire. A survey was conducted in the major production zones in Côte d’Ivoire to assess the incidence, severity, and distribution of cassava viral diseases. At each survey site, up to ten plants were assessed for symptom severity; incidence and samples were taken for virus testing. Techniques based on polymerase chain reaction (PCR) were used for the detection of cassava mosaic begomoviruses (CMBs) in the sampled leaves. Incidence of CMD varied from 0 to 100% and symptom severity from 1 to 5. Incidence differed significantly between the various agro-ecological zones (P<0.001), but severity was the same in those zones. Out of the 335 samples tested, African cassava mosaic virus (ACMV) was detected in 43.3%, East African cassava mosaic Cameroon virus (EACMCV) in 5.7%, and both ACMV and EACMCV in 31.3%; 19.7% of the samples analyzed were negative to all the viruses tested. None of the samples was tested positive to the East African cassava mosaic virus-Uganda (EACMV-Ug). These results suggest high incidence of CMD in the cassava production zones in Côte d’lvoire and underscores a need for implementation of control measures including phytosanitary measures with utilization of CMD-free materials for planting and adoption of resistant varieties. Get more articles at: http://www.innspub.net/volume-4-number-6-june-2014-ijaar/

See on figshare.com

[jbesinnspub]

Phytoextraction of salts by indigenous weeds in arid land

See on Scoop.it - Education

Reclamation of saline soil is becoming one of the most requirements for boosting crop production and sustainable agriculture. This study was conducted to investigate the capacity of three indigenous weeds growing naturally in arid land on west of Saudi Arabia. Salts content and biomass production for each weed species was evaluated. Salts concentration at three depths 0-10, 10-20 and 20-30 cm was estimated at growing and none growing sites for each weed species. The salts content for the weeds was found to be 6.26%, 16.80% and 5.83 % for Setariaverticillata, Dipterygiumglaucum and Tribulusterrestris respectively. Biomass production based on the dry weights was 13.37, 9.24 and 5.63 t ha-1 respectively. The removal efficiency was varied according to soil depth and initial soil salinity concentration. Get more articles at: http://www.innspub.net/volume-4-number-6-june-2014-ijaar/

See on figshare.com

[jbesinnspub]

Effect of gradual inclusion of papua foxtail millet (Setaria italica sp) to substitute yellow corn on breast meat quality and blood profiles in broilers

See on Scoop.it - Education

An experiment was carried out to examine effect of gradual inclusion of Papua foxtail millet (Setaria italica sp) to substitute yellow corn on breast meat quality and blood profiles in broilers. Two hundred and fifty day old chicks were used and alloted to 25 experimental units of 10 chicks. Five different dietary treatments were set, namely (i) T0 = basal feed, (ii) T1 = basal feed of which 2.5 % of yellow corn was substituted by Papua foxtail millet, (iii) T2 = basal feed of which 5.0 % of yellow corn was substituted by Papua foxtail millet, (iv) T3 = basal feed of which 7.5 % of yellow corn was substituted by Papua foxtail millet, (v) T4 = basal feed of which 10.0 % of yellow corn was substituted by Papua foxtail millet. All dietary treatments were set as iso-protein and iso-energy. At the end of experiment 2 birds in each experimental unit was selected, 25 birds were slaughtered and breast meats were sampled. Blood samples were taken from another 25 birds. On the basis of meat quality, water content, water holding capacity, colour and tenderness were analyzed. While for blood profiles, the measured variables included concentrations of serum haemoglobin, erythrocite, glukose and triglyceride. The results showed that level of Papua foxtail millet (Setaria italica sp) did dose dependently improve (P>0.05) tenderness of broiler meat, on the other hand redness of broiler meat did significantly change (P<0.01) with tendency less redness colour appear when Papua foxtail millet added more than 5% in the diet. No changes in lightness and yellowness of breast meat were reported. All blood profiles measurements of broiler showed that no physiologycal changes due to inclusion of Papua foxtail millet to replace corn. Based on this research, it is concluded that Papua foxtail millet might be used 10% in the diet to improve tenderness and redness of broiler meat. Get more: http://www.innspub.net/volume-4-number-6-june-2014-ijaar/

See on figshare.com

[jbesinnspub]

Effect of gradual inclusion of papua foxtail millet (Setaria italica sp) to substitute yellow corn on breast meat quality and blood profiles in broilers

See on Scoop.it - Education

An experiment was carried out to examine effect of gradual inclusion of Papua foxtail millet (Setaria italica sp) to substitute yellow corn on breast meat quality and blood profiles in broilers. Two hundred and fifty day old chicks were used and alloted to 25 experimental units of 10 chicks. Five different dietary treatments were set, namely (i) T0 = basal feed, (ii) T1 = basal feed of which 2.5 % of yellow corn was substituted by Papua foxtail millet, (iii) T2 = basal feed of which 5.0 % of yellow corn was substituted by Papua foxtail millet, (iv) T3 = basal feed of which 7.5 % of yellow corn was substituted by Papua foxtail millet, (v) T4 = basal feed of which 10.0 % of yellow corn was substituted by Papua foxtail millet. All dietary treatments were set as iso-protein and iso-energy. At the end of experiment 2 birds in each experimental unit was selected, 25 birds were slaughtered and breast meats were sampled. Blood samples were taken from another 25 birds. On the basis of meat quality, water content, water holding capacity, colour and tenderness were analyzed. While for blood profiles, the measured variables included concentrations of serum haemoglobin, erythrocite, glukose and triglyceride. The results showed that level of Papua foxtail millet (Setaria italica sp) did dose dependently improve (P>0.05) tenderness of broiler meat, on the other hand redness of broiler meat did significantly change (P<0.01) with tendency less redness colour appear when Papua foxtail millet added more than 5% in the diet. No changes in lightness and yellowness of breast meat were reported. All blood profiles measurements of broiler showed that no physiologycal changes due to inclusion of Papua foxtail millet to replace corn. Based on this research, it is concluded that Papua foxtail millet might be used 10% in the diet to improve tenderness and redness of broiler meat. Get more: http://www.innspub.net/volume-4-number-6-june-2014-ijaar/

See on figshare.com

[jbesinnspub]

Growth performance of spring wheat under heat stress conditions

See on Scoop.it - Education

Three spring wheat varieties and one advanced line were evaluated under high temperature environment to find out the heat stress tolerant variety(s) and/or genotype suitable for cultivation in rising temperature all over the Bangladesh, even in the world. The field experiment was conducted with two environments; one was normal growing environment (seeded on 30 November) and another in late seeding (heat stress) environment (seeded on 30 December) at the field of Wheat Research Centre, Bangladesh Agricultural Research Institute, Nashipur, Dinajpur, Bangladesh. The varieties and advanced line phased higher temperature during late seeding condition compared to normal sowing. The advanced line and varieties performed better in normal growing environment compared to heat stress condition. In stress environment, BARI Gom-27 produced the maximum tillers (706.5, 503.2 & 296.5 m-2 at 40, 60 & 80 DAS, respectively) followed by BAW-1151 and BARI Gom-28 produced the second highest at 80 DAS (287.4 m-2). BAW-1151 performed the best concerning dry matter (631.3 g m-2 at 80 DAS), then BARI Gom-28 (622.9 g m-2 at 80 DAS). In addition, BAW-1151 produced the most leaf area (3536.7 cm3 m-2 at 80 DAS) followed by BARI Gom-28 (3199.7 cm3 m-2 at 80 DAS). Moreover, BARI Gom-28 produced the highest yield in heat stress environment (3.59 t ha-1) followed by BARI Gom-27 and BARI Gom-26 (each 3.08 t ha-1), but BAW-1151 had the worst (2.9 t ha-1). Considering overall performance, the BARI Gom-28 can be the prominent variety for heat stress followed by BARI Gom-27 or BARI Gom-26. Get more: http://www.innspub.net/volume-4-number-6-june-2014-ijaar/

See on figshare.com

[jbesinnspub]

Nitrogen fertilizer and EDTA effect on Cannabis sativa growth and Phytoextraction of heavy metals (Cu and Zn) contaminated soil

See on Scoop.it - Education

Heavy metals in soil, water and air is a great concern. Toxic heavy metals ultimately affect plant, Animals, and through food chain directly influence human life. In present study, the effect of heavy metals (Cu, Zn), Nfertilizers and EDTA on growth and biomass of cannibus sativa plant was evaluated. The Cu and Zn phytoextraction potential of Cannabis sativa plant under various treatments of N-fertilizers and EDTA were investigated. Metals (Cu, Zn) significantly reduced the plant growth and biomass while the fertilizer application increased the plant growth and biomass under metals stress. The application of EDTA alone increased the metals (Cu, Zn) accumulation in root, stem and leaves but reduced the plant height, root length and biomass. The maximum accumulation of Cu in root (75 μg/g DW), stem (55 μg/g DW) and in leaves (45 μg/g DW) was found with EDTA treatment (T3). Maximum Zn concentration in Leaves (155 μg/g DW) and root (148 μg/g DW) was observed in plants treated with EDTA. Conclusively, metals contaminated soil considerably reduced plant growth and biomass while metals in combination with N-Fertilizer the plant growth and biomass was enhanced. Addition of EDTA significantly enhanced phytoaccumulation of metals (Zn, Cu). Get more articles at: http://www.innspub.net/volume-4-number-6-june-2014-ijaar/

See on figshare.com

[jbesinnspub]

Growth enrichment of Carthamus tinctorius (L) and reduction in dosage of chemical fertilizers with application of plant growth promoting rhizobacteria

See on Scoop.it - Education

Experiencing the adverse effects of synthetic chemical fertilizers in agriculture production the concept of biofertilizers has gained interest currently. The aim of the current study was to evaluate the integrated role of plant growth promoting rhizobacteria (PGPR) and chemical fertilizers on growth and yield attributes of safflower during growing season of 2009-10 and 2010-11. The chemical fertilizers were applied at full (Urea 60 Kg ha-1 and DAP @ 30 Kg ha-1), half (Urea 30 Kg ha-1 and DAP 15 Kg ha-1) and quarter doses (Urea 15 Kg ha-1 and DAP 7.5 Kg ha-1). The PGPR (Azospirillum brasilense and Azotobacter vinelandii) were applied as seed inoculation @106cells/ml. Maximum plant height, number of branches/plant, leaf chlorophyll and leaf area was recorded in Azospirillum and Azotobacter with full dose of chemical fertilizers and with full dose of chemical fertilizers treatments alone while maximum number of capitulum/plant, capitulum weight and seed yield was shown by Azospirillum brasilense supplemented with half dose of chemical fertilizers. Maximum achene oil contents were recorded in Azospirillum treatment supplemented with half dose of chemical fertilizers and Azotobacter+full dose of chemical fertilizers. The PGPR improved plant growth and yield by reducing the use of chemical fertilizer (50 %), hence leading towards sustainable agriculture production. See more: http://www.innspub.net/volume-4-number-6-june-2014-ijaar/

See on figshare.com

[jbesinnspub]

Biocontrol of Rhizoctonia solani causing stem canker disease of potato using rhizosphere mold fungi

See on Scoop.it - Education

The aim of the present work is to obtain effective rhizosphere fungal bio-control agents (bio-fungicides) that can antagonize Rhizoctonia solani; the fungal causal agent of stem canker disease of potato (Solanum tuberosum L.) and to improve the growth of the potato plant. A total of eleven different isolates of mold fungi were isolated from the rhizosphere soil of potato plants collected from three different potato cultivation areas in El-Monofia governorate-Egypt. Three isolates belong to the genus of Fusarium and three to Aspergillus, whereas the other five isolates belong to the genera of Penicillium, Absidia, Mucor, Gliocladium and Alternaria. In dual culture technique on Sabouraud dextrose agar media, three species (Gliocladium virens; Penicillium oxalicum and Trichoderma viride), completely surrounded the pathogen colonies and prevented their radial spread. In vivo results of antagonism in greenhouse were very promising as growth of potato plant was highly promoted by the three bio-agents compared with positive and non-infested control plants. Studies on soil population dynamics of selected Gliocladium virens showed a gradual decrease in CFU with increasing periods of incubation. Maize kernels and wheat bran were the best solid substrates for the mass production of Gliocladium virens conidia especially at 30% initial moisture level. The highest percentage of viable conidia of formulated Gliocladium virens was obtained on storage at 4˚C. Gliocladium virens showed promising results of antagonizing R. solani in vitro and improving potato growth under greenhouse conditions, subsequently, could be mass produced and formulated to be used as an effective biofungicide. See more: http://www.innspub.net/volume-4-number-6-june-2014-ijaar/

See on figshare.com

[jbesinnspub]

Foraging and pollination behavior of Apis mellifera adansonii Latreille (Hymenoptera, Apidae) on Brachiari brizantha (Hochst. Ex A. Rich.) Stapf. 1919 flowers at Dang (Ngaoundere- Cameroon)

See on Scoop.it - Education

To evaluate the Apis mellifera adansonii Latreille (Hymenoptera: Apidae) impact on fruit and seed yields of Brachiaria brizantha H. (1919) (Poaceae), A. m. adansonii workers foraging and pollinating activities were studied in Ngaoundéré. From September to October 2010 and from September to October 2011, the experiments were carried out on 240 and 440 inflorescences divided in three lots: two lots differentiated according to the presence or absence of protection regarding A. m. adansonii and other insects visits; the third protected and uncovered when flowers were open, to allow insect visits. Worker’s seasonal rhythm of activity, its foraging behavior on flowers, its pollination efficiency, the fructification rate and the percentage of normal seeds were evaluated. Results show that A. m. adansonii foraged on B. Brizantha flowers throughout the whole blooming period. This bee intensely and preferably harvested pollen. The greatest mean number of individuals foraging simultaneously on 1000 flowers was 10 in 2010 and 625 in 2011. The mean duration of a visit per spikelet was 5.77  5.08 sec in 2010 and 4.94  3.44 sec in 2011. The mean foraging speed was 4.55 flowers/min in 2010 and 6.86 flowers/min in 2011. The fructification rate and the percentage of normal seeds of unprotected inflorescences were significantly higher than those of inflorescences protected from insects. Through its pollination efficiency, A. m. adansonii provoked a significant increment of the fructification rate by 37.70 % in 2010 and 34.75 % in 2011, as well as the percentage of normal seeds by 3.39 % in 2010 and 6.82 % % in 2011. The installation or conservation of A. m. adansonii nests close to B. brizantha fields could be recommended to increase seeds and fruits production in the region. Get more: http://www.innspub.net/volume-4-number-6-june-2014-ijaar/

See on figshare.com

[jbesinnspub]

Molecular and physiological responses of Pisum sativum and Vicia faba to sodium azide

See on Scoop.it - Education

Seeds of Pisum sativum and Vicia faba were pre-soaked in either 1.0, 2.0 or 4.0 mM of sodium azide (NaN3) for 30 min, 1 h or 2 hs then, sown in clay soil in plastic pots under conditions of 25/18°C day/night and natural light (16/8 h day/night) and the seedlings were harvested after 21 days. The results revealed that total phenols, H2O2, malondialdehyde and the activities of catalase, peroxidase and DPPH scavenging activity in both plant were gradually increased with the increase in NaN3 concentration and soaking time. The isozymes of α- and β- esterases and α-amylase had number of loci ranged from two (α-amylase) to four (α-esterase) in pea, while in bean, it was three for the three investigated enzymes. The ISSR analysis revealed that both plants showed highly significant increase in the appearance of bands by the highest treatment of NaN3, especially with the longest duration. Pea produced 14 polymorphic, 9 monomorphic and 4 unique bands, while bean produced 14 polymorphic, 8 monomorphic and 3 unique bands.Get the full articles at :http://www.innspub.net/volume-4-number-6-june-2014-ijaar/

See on figshare.com

[jbesinnspub]

Application of biophysical and biochemical methods as priming techniques on Carthamus Tinctorius L.

See on Scoop.it - Education

For disclosing the effects of seed priming with biophysical and biochemical methods on yield and other characteristics on safflower (Carthamus Tinctorius L.), the seeds of one safflower cultivar was treated with 72 mT strength for 10 min, hydro priming for 72 hours and gibberellic acid with 50ppm concentration for 8hours before germination and cultivation. In this experiment traits such as yield components, seed yield, biological yield, harvest index, petal yield, percentage of oil, percentage of huld seed and correlations were measured. Seed priming was significantly impressed characteristics under study. Plants showed that yield parameters and major traits were increased, in most cases, for magnetic treatment versus control (non-treated seeds) and other pretreatments. Results indicated that biophysical methods (magnetic field) had greatest difference with biochemical methods and control at traits under study. Get more articles: http://www.innspub.net/volume-4-number-6-june-2014-ijaar/

See on figshare.com

[jbesinnspub]

figshare

See on Scoop.it - Education

For disclosing the effects of seed priming with biophysical and biochemical methods on yield and other characteristics on safflower (Carthamus Tinctorius L.), the seeds of one safflower cultivar was treated with 72 mT strength for 10 min, hydro priming for 72 hours and gibberellic acid with 50ppm concentration for 8hours before germination and cultivation. In this experiment traits such as yield components, seed yield, biological yield, harvest index, petal yield, percentage of oil, percentage of huld seed and correlations were measured. Seed priming was significantly impressed characteristics under study. Plants showed that yield parameters and major traits were increased, in most cases, for magnetic treatment versus control (non-treated seeds) and other pretreatments. Results indicated that biophysical methods (magnetic field) had greatest difference with biochemical methods and control at traits under study. Get more articles: http://www.innspub.net/volume-4-number-6-june-2014-ijaar/

See on figshare.com

[jbesinnspub]

Extraction of β-galactosidase and β-glucosidase from the seeds of Tamarindus indica

See on Scoop.it - Education

The enzymes β–galactosidase and β–glucosidase were extracted from the tamarind seeds using different buffers at different pH. Highest activity was obtained with 10 mM sodium acetate buffer, pH 5.6 and 10 mM tris buffer, pH 7.4. The effect of NaCl and Triton X–100 at different concentrations on the extraction of the enzymes indicated 10 mM sodium acetate buffer, pH 5.6 containing 1 M NaCl as a better extractant of the enzyme. The enzyme assay was carried out using p–nitrophenyl– β–D–galactoside and p–nitrophenyl–β–D–glucoside as substrates. Highest enzyme activities were observed on 6th and 24th day of germination. The protein content gradually decreased upto 5th day of germination and suddenly increased on 6th day. However, on subsequent days of germination, the protein content greatly decreased upto 11th day. During the latter period of germination (18th day onwards) the content remained almost constant. The kinetic parameters varied for both β–galactosidase and β–glucosidase. The activity of β–galactosidase was show to have an optimal operating condition at pH 5.5 and a temperature of 500C. The thermostability of the enzyme was in the range of 400C – 700C with the pH stability in the range of 5.0 – 7.0. The Km and Vmax values for pNPGal were determined as 66μM and 2.27nmolesmin-1. In contrast the activity of β–glucosidase was shown to have an optimal operating condition at pH 5.0 and a temperature of 300C. The thermostability of the enzyme was in the range of 270C – 350C with the pH stability in the range of 4.0 – 7.0. The Km and Vmax values for pNPGlu were determined as 121μM and 5.26nmolesmin-1. The presented study is a preliminary work carried out for the standardization of protocols. The purification and characterization of β–galactosidase and β–glucosidase is under progress. See more articles: http://www.innspub.net/volume-1-number-3-november-2011/

See on figshare.com