ELISA -Kit for Detection of Cucumber Mosaic Virus Description Polyclonal antibodies (PAbs) to recombinant nucleocapsid protein (rCP) ofCucumber mosaic virus (CMV) were used in ELISA to detect both the subgroup CMV in crude extract. The conserved CP gene of CMV was cloned and an expression construct was developed in pET vector. rCP was expressed in E. coli and purified. PAbs were generated against rCP for broad spectrum detection of viruses. Enzyme-linked immunosorbent assay (ELISA) kit has been developed to detect CMV viruses. Salient Feature Indigenous diagnostic antibodies to plant viruses are not commercially available in India. The major limitation in the production of antibodies is in the purification of adequate quality and quantity of antigen (native virus particle) from plant tissues. We have overcome this limitation by developing nucleocapsid protein expression construct, which can be used for the production of unlimited quantity of antigen facilitating production of polyclonal antibodies, the key reagent in ELISA kit, at the commercial scale. Approximately, the antibodies can be raised by two months time. The technology will benefit entrepreneurs to produce indigenous commercial kit. Diagnostic kit will help decision making on judicial application of insecticides for the control of CMV in various crops. The diagnostic kit will help in developing resistant cultivars. (at Delhi) https://www.instagram.com/p/BswoquPHUCC/?utm_source=ig_tumblr_share&igshid=nlg8r2q29dd4
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