Nature of Nature

images of nature expression

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Leishmania major promastiotes during infection of primary fibroblast culture. Cells are stained with anti-tubulin (green) and anti-actin (red).

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Confocal microscopy of plant tissues

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Pilidium larva of the nemertean Cerebratulus sp.

Labelled with phalloidin (green: actin) and DAPI (blue: nuclei).

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This microscopic fluorescent image of the mosquito's circulatory system shows muscle cells in green; cellular DNA in blue and periostal hemocytes in orange.

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Confocal micrograph of a blind cavefish embryo at around five days post-fertilisation viewed from the side (lateral view) with an antibody that targets a calcium binding protein (calretinin) shown in green, which highlights different neuronal types and their processes in the nervous system. The cavefish Mexican tetra (Astyanax mexicanus) has a seeing and a blind form; the latter lives in dark environments, and relies on other senses. The blind cavefish has specially adapted traits that its sighted relation (dwelling near the surface) does not. These include a greater number of sensory receptors and taste buds along its body; these taste buds are also more efficient than the equivalent cells in the seeing cavefish. The eyes are still present at this stage of development but they will degenerate naturally during the lifetime of the fish as they live in a dark environment where eyes are redundant. Adult cavefish are blind.

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Sensory axons (long, slender nerve fibers) covering the tail of a 3- day-old larval zebrafish. This "Brainbow" image was collected using confocal microscopy. In the Brainbow technique (Nature, 2007), cells randomly choose combinations of red, yellow and cyan fluorescent proteins, so that they each glow a particular color. This provides a way to distinguish neighboring cells of the nervous system and follow their pathways. Seventh Prize, 2009 Olympus BioScapes Digital Imaging Competition

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new tattoo :)

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L. polyphemus, Cast skin of a horseshoe crab Cuticular receptors Autofluorescence, Ex 488, Det 500-600 nm, Color Coded Projection Dr. Manfred Schmidt University of Hamburg, Zoological Institute, Neurophysiology, Martin-Luther-King-Platz 3, 20146 Hamburg

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Confocal image of Membranipora membranacea. The triple staining shows three organs in one image. Cells of the nervous system are indicated in pink, ciliated organs in blue, muscular structures in green.

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Ferroelectric liquid crystal domains nucleating from isotropic liquid of P10PIMB (known as bent-core liquid crystal material) (120x)

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Cell infected with poxvirus (630x)

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Spirorbis sp. (aquatic worm) (100x)

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2-Photon fluorescence micrograph of cell nuclei of the mouse colon(740x)

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Winner of 2013 Drosophila Image Award A complex set of muscles underlying the body wall of third-stage Drosophila larva

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Multiplex imaging of apoptosis. U2OS cells were treated with 30 μM etoposide for 18 hr to induce apoptosis. The treated cells were stained first with 7.5 μM CellEvent Caspase-3/7 Green detection reagent (green fluorescence, C10423) to detect apoptosis and Hoechst 33342 nucleic acid stain (blue fluorescence, H3570) to label nuclei, and then with 150 nM MitoTracker Deep Red FM (pink fluorescence, M22426) to label mitochondria. Following fixation and permeabilization, actin was labeled with Alexa Fluor 546 phalloidin (orange fluorescence, A22283).

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Tetrahymena from outside and inside :)

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A human HaCat keratinocyte responds to epidermal growth factor by rapidly forming a lamellipod around most of its perimeter. The cell was fixed and processed within minutes after EGF addition. F-actin is stained with fluorescently labeled phalloidin (light purple), and microtubules are labeled with an antibody (cyan). DNA dye stains the nucleus dark purple.