Use of Safe-Lock 1.5 & 2ml tube
1.5ml and 2ml tubes are like basic materials for any biology labs but safe locked 1.5ml & 2ml tubes are extensively used in molecular biology labs. In most of the cases, we used a 1.5 ml tube and it's well served all purposes. Sometimes if the volume is not sufficient then only we prefer a 2ml tube. But here I am going to share my experience regarding the use of safe locked 1.5 and 2ml tube that has a great impact when you are dealing with low quantity of DNA/RNA.
The major physical differences between these two are the bottom of the tube which is conical for 1.5ml and round for 2ml. This round-shaped bottom of the 2ml tube increases the probability of not losing the pallet during the precipitation step. After centrifugation when you will proceed to throw the supernatant, It is most likely to end up with some amount of alcohol in a 1.5ml tube due to its conical bottom. So, after throwing the supernatant when you will straight the tube to see the pallet; high chances are there that it will dissolve again with that remaining amount of alcohol if concentration is less. This pallet is difficult to detect due to smaller in size and more transparent. These things you can avoid in a 2ml tube due to the round bottom. It always works for me. Hope it will work for you also. All the best.
