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Abstract The analysis of nucleotide sequences of the Argemone genus samples by bioinformatics methods was aimed at the study of phylogenetic
Abstract The analysis of nucleotide sequences of the Argemone genus samples by bioinformatics methods was aimed at the study of phylogenetic relationships of species within the genus, at the development of genus-specific primer pairs, and TaqMan-probe and their verification in silico. Molecular genetic studies by the polymerase chain reaction method in the “real-time” mode (RT-PCR) were devoted to checking the “performance” and specificity of the developed system of primers and TaqMan-probe using samples of eight species of Argemone and 11 species of other plants. Phylogenetic analysis using internal transcribed spacer 1 (ITS1) sequences confirmed previous phylogenetic studies and improved understanding of relationships within the genus. Before the in vitro test of the developed system of primers and TaqMan-probe, the ability of the extracted DNA to be amplified by the RT-PCR method using the system of primers and the TaqMan-probe to the 18S rRNA gene of eukaryotes was checked to exclude pseudo-negative results during the further verification of specificity. The conditions for RT-PCR were selected, namely, temperatures and times of denaturation, hybridization, elongation, and the number of cycles. The developed system of primers and TaqMan-probe for the RT-PCR method demonstrated high species specificity and sensitivity. Amplification was noted only in DNA samples of eight Argemone species, while PCR analysis of other plant species and negative controls showed no amplification. The detection limit of the developed system was determined to be 0.01%. It is proposed that this marker system be used to detect falsification in food and other products, particularly Argemone contamination in mustard or coconut oil.
Argemone polyanthemos / Thistle Poppy on the Red Rocks Trail in Lakewood, CO
सत्यानाशी I Argemone_mexicana I Mexican_poppy_Remedy_Piles_Asthma_Pimples_Immunity_Arthritis_DIY
Safer-Effective-Better-Herbal_Remedies
#सत्यानाशी #Argemone_mexicana #Mexican_poppy #Remedy #Piles #Asthma #Pimples #Immunity_Arthritis_DIY #Wound_healer #Gall_bladder #Emetic #toxins #Cholesterol #Asthma #pruritus #lungs #urinogenital_disorders #veneral_disease #anxiety #Urolithiasist #diy #papaveraceae #nature #naturelovers #anxiety #striped #healthylifestyle #liver #kidneys #brain #ornamental_plant #diabetes #stomach_ulcer…
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🌱Le pavot mexicain ou argemone du Mexique est une plante annuelle piquante dont la floraison est jaune en été. Cette plante médicinale à la particularité d'améliorer la qualité du sommeil. Graines d'argemone à cultiver actuellement disponibles sur le site ethnoplants. #argemone #argemonamexicana #sedatif #dormir #pavot #pavotmexicain #mexicanpoppy #poppy #opiomexicana #argemoneproducciones #argemona #plantemedicinale #jardin #jardinage #jardiner #semis #planter #graine #plante #semence #permaculture #potager #potagere https://www.instagram.com/p/CnKkQ9wKo27/?igshid=NGJjMDIxMWI=
Argemone glauca, or Pua kala, is one of the prickly poppies (family Papaveraceae) and is endemic the Hawaiian islands where it exists at many altitudes. A. glauca (named for the glaucous = gray green/blue leaves) produces a classic white flower with yellow center and has spiny leaves which along with toxic alkaloids deter herbivores from grazing. While not possessing the opioids for which poppies may be best known, the alkaloids Pua kala possesses made it useful to native Hawaiians for treating various medical conditions such as ulcers, toothaches, and warts. Like its mainland counterparts, A. glauca prefers fairly dry habitats, doing well in places with little rainfall. Though native to only north and south America, various species of Argemone have been introduced throughout the world, including different species though within the native range of the genus. Argemone mexicana is a fairly close relative of A. glauca and has been introduced to the Hawaiian islands, benefiting from adaptation to similar environments.
Argemone munita
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