#biiology

[jordans-studyblr]

hey guys, im still a very new studyblr, and i need people to follow!!! if you post any of the following content, please reblog/like this post so i can follow you!!

biology

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[shycollectorcat]

You are studying the effect of a new pollutant on an ecological - 00398825 Tutorials for Question of Biology and General Biology

You are studying the effect of a new pollutant on an ecological - 00398825 Tutorials for Question of Biology and General Biology

[letsgomaybe]

April 7

We read a paper today that led to a fun discussion about PCR bias and how we’d see other communities if we sampled them the same way as bacteria and other tiny organisms. Like, you can’t just count bacteria like you can plants and animals. What most people do is amply the 16S region and cluster the sequences together in Operational Taxonomic Units (OTUs) which serve as a proxy for genera. But different sequences can undergo PCR at different rates, so you get a whole bunch of DNA from the one that PCRs the fastest. It’s definitely not a good metric to describe the abundance of bacteria within a community. With the newest sequencing technology it’s slowly becoming possible to sequence without much PCR (PacBio metagenomics) so hopefully this won’t be a huge problem for much longer. I learned stuff today!