Maize (Zea mays L.), a globally important cereal crop, is susceptible to numerous diseases, with Maydis Leaf Blight (MLB) caused by Bipolaris maydis posing a significant yield threat. This study investigated cytogenetic behavior, pathogen identification, and inheritance of MLB resistance in a maize × teosinte (Zea mays ssp. mexicana) crosses. The experiment was conducted in the subtropical Gangetic region under natural MLB incidence. F₂ populations (n=381) derived from an agronomically superior but susceptible maize parent and a resistant teosinte parent were evaluated. Cytological analysis of F₁ pollen at metaphase I revealed irregular pairing, with 9II+2I or 8II+4I configurations, indicating partial chromosomal homology between parents. Pathogen isolation from symptomatic F₂ leaves on potato dextrose agar confirmed B. maydis through colony morphology and characteristic olivaceous brown, spindle-shaped, multi-septate conidia. Disease scoring (0 to 5 scale) showed 58.53% resistant (score 1), 33.07% moderately resistant (score 2), and minimal susceptibility. Chi-square analysis of resistance vs. susceptibility (score ≥2) fit a 9:7 ratio (p
