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Countstar® Rigel S5 Advanced Image Cytometer

PBMC and Primary Cell Counting

Cell Apoptosis

Cell Cycle

Surface Marker Analysis

GFP Transfection Efficiency

FCS Express Software

Product Overview

The Countstar Rigel S5 system increases the PE channel on the basis of Rigel S3, and can carry out multi-color fluorescence experiments in addition to rapid cell viability and concentration measurement, including cell apoptosis (Annexin V/PI/DAPI), cell transfection (GFP/YFP/RFP), CD marker experiment (DAPI/FITC/PE), and cell killing (Hoechst T33342/Calcein-AM/PI) and so on.

Excitation light: 375nm, 480nm, 525nm

Emission filter：460nm, 535nm, 580nm, 600LP

Key Benefits

All in one design- Integrated Computer/Touch scree

Automatic Detection 5 Samples in one single test sequence.

User Friendly Cell Analysis Protocols

Unrivaled "Fixed focus" patent, no manual focusing.

High Stability and Actuary

Powerful and user-friendly Data Management

GMP and 21 CFR part 11 ready

FCS Express Software

Product feature

SmartThe BioApps of the Countstar Rigel S3 System simplify routine cell lab tasks while providing high quality scientific data.

Countstar S3 comes with three BioApps to simplify and automate the routine cell count, viability and cytotoxicity tasks.

AO/PI Viability Protocol: Run two fluorescence color assays in disposable consumable to determine the percentages of live, dead cells and concentration in the presents of debris and unwanted nonnucleated cell types including red blood cells.

Affinity of Antibody: This assay run as Green color assays in FITC binding antibody to determine the affinity of the biosimilar drug.

Surface Marker Assay: Quantify specific cell populations based on surface marker expression (CD45+ CD4+, CD8 + MSC, CD56+ NK cells, etc.)

Cell Cycle: Propidium iodide (PI) is a nuclear staining dye that is frequently applied in measuring cell cycle. The protocol determining cellular DNA content in cell cycle analysis

Cell Apoptosis: Cell Apoptosis assay is a type used for determining the apoptosis percentage of cells by Annexin V-FITC/7-ADD staining method.

GFP transfection: The green fluorescent protein (GFP) exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. This protocol can analyze counting and percentage of GFP.

Celling Killing: Run three fluorescence color assays in disposable consumable to determine the CAR T/NK-Mediated Cytotoxicity using Tracer and Viability Dyes

Trypan blue Protocols: Obtain cell count, viability and concentration estimations based on trypan blue staining using a disposable consumable.

Three step process

The Countstar S3 System is designed to take you from sample to result in one go, simplifying your work and allowing for increased productivity and efficiency.

Reproducible and Accurate

Countstar S3 system can automatic detect 5 samples at one time, it will be more efficiency and affordable, with integrated patent “fixed focus”, the results would be more stable and reliable.

Touch Screen with Flexible Experiment Assay Management System

“App-like" interface. Allow to add multiple Experiment Assays for different operators

FCS Express Software

The optional De NovoTM FCS Express software makes graphs touchable and customizable fluorescence channel boost your experiment reach. Countstar Rigel S5 together with FCS Express is able to analyze for the cell apoptosis, cell cycle, transfection, affinity of antibody, CD marker and etc.

GMP ready

The Countstar S5 system is designed to meet the modern pharmaceutical requirements. The software complies with 21 CFR part 11. Key features include control of user access and data export, and secure audit trail. At the same time, we also supply IQ/OQ service and PQ support to help customer build a reliable system.

Specifications

Technical Specifications

Model:

Countstar Rigel S5

Diameter range:

3μm ~ 180μm

Concentration range:

1×104 ~ 3×107/mL

Objective magnification:

Imaging element:

1.4 megapixel ,CCD camera

Excitation Light

375nm, 480nm, 525nm

Emission Filter

460nm, 535nm, 580nm, 600LP

USB

1×USB 3.0 1×USB 2.0

Storage:

500GB

Power supply:

110–230 V/AC, 50/60Hz

Screen:

10.4 inch touchscreen

Weight:

13kg (28lb)

Size (W X D X H):

Machine: 254×303×453mm Package size: 430×370×610mm

Operating temperature:

10℃ ~ 40℃

Working humidity:

20% ~ 80%

Applications Dual-Fluorescence Viability

Acridine orange (AO) and Propidium iodide (PI) are nuclear nucleic acid binding dyes. The analysis excludes cell fragments, debris and artifacts particles as well as undersized events such as platelets, giving a highly accurate result. In conclusion, theCountstarsystem can be used for every step of the cell manufacturing process.

T/NK cell mediated cytotoxicity

By labeling the target tumor cells with CFSE ortransfectwith GFP. Hoechst 33342 is used for stain all cells (both T cells and tumor cells), alternatively, target tumor cells can be stained with CFSE, PI is used forstainthe dead cells (both T cells and tumor cells). This staining strategy allows for the discriminate of different cells.

Cell Apoptosis

The different phases of apoptosis are segmented into an early, middle and late stage. Specified indicators can document the single stages of apoptosis. The Countstar® Rigel S5 offers a visualized image-based solution and analyzes all of these steps with pre-loaded protocols in depth.

Cell Cycle

CountstarRigel S5 enables users to get the result of a cell cycle quickly and accurately.CounstarRigel S5 can analyzeproportionof cell indifferentphase of the cell cycle.

Surface Maker Analysis

A lymphocyte subsets analysis is a typical experiment performed incell relatedresearch fields and various diseases diagnosis. Countstar® Rigel S5 offers a faster and easier way to make immune cell typing more efficient. With visible cell images and powerful data analysis

Affinity of antibody

Detect affinity of antibody in cell level is an important indicator of monoclonal antibody detection using immunofluorescence method.TheCountstar® Rigel S5 offers a rapid, direct and reliable evaluating method for the affinity of antibody detection in antibody drug screening

GFP Transfection Efficiency

In cell and molecular biology, the GFP gene is frequently used as a reporter of expression. Currently, scientists are commonly using the fluorescent microscopes or flow cytometers to analyze the transfection efficiency of mammalian cells. But Flow cytometry requires a high-qualified and experienced operator. WhileCountstarRigel S5 enables users to get the result of a transfection efficiency assay quickly and accurately.

#ALIT Life Science #AO/PI #Cell Counter #Cell Cytotoxicity #Countstar #Trypan Blue

Countstar® BioTech Automated Cell Counter

Accurate cell analysis for:

Trypan blue Cell Counting

Aggregated Cell Analysis

Cell Diameter Analysis

Cultivation time chart (CTC)

Product Overview

The Countstar® is designed based on the classic trypan blue staining for cell viability principle, integrating the advanced “fix focus” optical imaging work bench and the most advanced cell recognition technologies and software algorithms. Countstar BioTech not only provides the cell concentration and viability, but also provides the average diameter and average circularity, and showing the diameter range and aggregation of cells in histograms etc.

Key Benefits

“Fixed Focus”– No manual focus adjustment needed

Fast testing time – less than 20s

Data easily comparable with a hemocytometer

Maximum loaded 5 samples in one slide

Accurate and high reproducibility result – Intra-assay & Inter-assay CV <5%

Maintenance and service free

Powerful software and data management System

IQ/OQ validation and PQ support

Product feature Easy and fast:

Within 20 seconds through three steps with one button

Cost-efficient Consumables:

Loaded 5 sample in one slide Individual Package

Imaging Technology:

5-megapixel color imaging and a wide sample collection range ensures clear and accurate visualization

Accuracy and Reliability:

Guaranteed by “Fixed Focus” technology, high resolution imaging, larger observation area and advanced algorithms.

Countstar VS Hemocytometer

Powerful data analysis and management System:

1. Intelligence search, Coded lock, security user login and sustainable data management etc., to keep the cell quality and data safety.

2. Countstar software system provides cultivation time chart (CTC), overlay analysis and other statistical and analytical functions

3. Diversity of Data Formats: PDF, EXCEL, JPEG；Automated PDF reports

4. Sustainable Data Management

User, Time, Parameter, Result……

Specifications

Technical Specifications

Model:

IC 1000

Test Item：

Concentration, Viability, Diameter, aggregation etc.

Sample Density：

1x104 - 3x107/ml

Sample Diameter：

5-180μm

Imaging element:

5 Megapixel，CMOS camera

Objective magnification:

2.5 X

Sample Volume：

20μL

Test Time：

<20s

Output：

JPEG/PDF/EXCE

Applications 1. Trypan blue cell counting and viability

Countstar is applicable to cells with diameter between 5-180um, like mammalian cell, insect cell, and some planktons.

2. Aggregated Cell Analysis

Some primary cells or subculture cells are prone to aggregate when poor culture state or excessive digestion, thus causing great difficulty in the counting of cells. With the Aggregation Calibration Function, Countstar® can realize a stimulation calculation of aggregations to ensure accurate cell counting and obtain the aggregation rate and the aggregation histogram, thus providing basis for experimenters to judge the state of cells.

Countstar can count the aggregated cells one by one.

3. Cell Size Analysis

The change of cell size is a key feature and is commonly measured in cell research. Normally it will be measured in these experiments: cell transfection, drug test and cell activation assays.

4. Overlay Analysis of the Cell Growth Curve

In cell culture, the study of pharmacology and toxicology often requires the overlay analysis of multiple growth curves in order to find the optimal culture conditions or the best dosage. Countstar® can directly call up multiple growth curves for comparative analysis.

#ALIT Life Science #Cell Counter #Countstar

Countstar® BioMed Professional Immune Cell Counter

1. Trypan blue Cell Counting

2. Immune Cell Counter

3. Cell Diameter Analysis

4. Cultivation time chart (CTC)

Product Overview

In order to meet the requirement of cell therapy institutes, RuiYu developed the Countstar BioMed. It is applicable to cells with diameters ranging from 2-180μm, and realizes an accurate and reliable counting of cells commonly seen in clinical treatment such as lymphocytes, dendritic cells and other stem cells. Providing a series of solution from cell counter, cell analysis, data storage and manage to data output.

Key Benefits

More Wider Test Range: Cell diameter: 2-180μm；

"Fixed Focus" – No manual focus adjustment needed

One equipment code specific to each measurement;

Powerful software and data management System;

E-signature and 4-level User Access Management;

IQ/OQ validation and PQ support

Product feature

Keeping the features of Countstar BioTech, Counstar BioMed provides the new characters as follow:

1.EPC-based Information System, storing the operation records all the time;

2. 5x magnification with wider test range: Cell diameter: 2-180μm；

3. Central data bank allows the user to keep all the data, like instrument test report, cell sample report and tester e-signature, safe and permanent.

4. level access user management system

5.EPC-based Information System, storing the operation records all the time;

6. The database supports sustainable management, and data can be traced through the sample No., operator and cell parameters;

Specifications

Technical Specifications

Model：

IM 1200

Test Item：

Concentration, Viability, Diameter, aggregation etc.

Sample Density：

1x104 - 3x107/ml

Sample Diameter：

2-180μm

Imaging element:

5 Megapixel，CMOS camera

Objective magnification:

5 X

Sample Volume：

20μL

Test Time：

<20s

Output：

JPEG/PDF/EXCE

Applications

Immune Cell Analysis

It is applicable to cells with diameters ranging from 2-180μm, and realizes an accurate and reliable counting of cells, such as lymphocytes, dendritic cells and other stem cells.

#ALIT Life Science #Cell Counter #Countstar #GFP Transfection #RFP Transfection

Countstar® BioFerm Automated Yeast Cell Counter

Countstar® BioFerm

Automated Yeast Cell Counter

Yeast Cell Count

Pitching Yeast

Brewing Yeast

Product Overview

The Countstar® BioFerm Automatic Yeast Counter combines traditional Melanie or Methylene Violet staining with high-resolution imaging and intelligent image recognition for accurate and consistent detection of yeast concentration and viability with a powerful Data Management System.

Key Benefits

Fast and easy operation, 20s for each sample

Dilution Free( 5x104 - 3x107 cells/ml )

Safe handling and disposal of samples with traditional stains such as Methylene Blue

Data easily comparable with a hemocytometer

Unique “Fixed Focus” image analysis provides reproducible data

Low cost and waste per test using disposable, each chamber slide with 5 chambers

Maintenance free

Product feature

Product Performance Testing

Diameter Measurement Comparison

Reproducibility test (25 samples, two staining methods for comparison with the hemocytometer)

Specifications

Technical Specifications

Model：

IY 1200

Test Item：

Concentration, Viability, Diameter, aggregation etc.

Sample Density：

1x104 - 3x107/ml

Sample Diameter：

2-180μm

Imaging element:

5 Megapixel，CMOS camera

Objective magnification:

5 X

Sample Volume：

20μL

Test Time：

<20s

Output：

JPEG/PDF/EXCE

Applications

The Countstar® BioFerm is able to accurately count the yeast cell with diameter between 2 to 180μm.

Example 1: Live Yeast Cell

Example 2: Dead Yeast Cell

#ALIT Life Science #Cell Counter #Cell counting #Countstar #Hemocytometer #yeast cell count #Yeast Counting