Viral RNA Extraction Kits for diagnostics associated with Coronavirus testing
Viral RNA Extraction Kits:
Viral RNA Extraction is a prerequisite for RT PCR analysis to test Coronavirus in asymptomatic, infected, and suspected cases. RNA Extraction needs to be done carefully to obtain high quality and quantity of RNA that can be used for further downstream applications. Low quality of RNA is not preferred. Therefore, RNA extraction kits are used in different labs working on COVID-19 testing. RNA Extraction kit price in India varies from one diagnostic lab to another. Some manufacturing and diagnostic companies are supplying Viral RNA extraction kits at an affordable price, while some are charging on a higher side depending upon the kits' automation and components. Using the kit, the process of RNA extraction becomes much more comfortable, and the high quality and quantity of RNA can be isolated. Using the kit, the time also gets reduced to isolate high-quality RNA.
Using Viral RNA Extraction Kits:
The top labs of the countries use viral RNA Extraction Kits for Coronavirus testing. The increasing number of cases is increasing the demand for more testing strategies. RT PCR tests are being preferred and are considered gold standard protocols for confirmation of Coronavirus in suspected and asymptomatic cases. RT PCR protocol requires good quality of viral RNA, which is usually isolated from the clinical specimen present in the viral transport medium. These clinical specimens are usually nasopharyngeal and oropharyngeal swabs.
In some cases, human blood and serum are also used as the samples for isolation of impurity-free RNA. RNA extraction kits form an essential aspect of COVID-19 testing kits as they form the most critical step in Coronavirus diagnostics. Viral RNA extraction kits should be used as per the protocol provided with the kit. The RNA may not be pure or may lack quality if the end-user does not follow proper protocol.
Principle of Viral RNA Extraction Kits:
Most of viral RNA extraction kits supplied by diagnostic manufacturers use magnetic nanoparticle technology. Magnetic beads are used for isolation of high quality and quantity of RNA. This technology helps recover more than 90-95% viral RNA from the clinical sample collected from the collection site. The kits can be used for manual and automated extraction of RNA using various machines. Some diagnostic companies are supplying carrier RNA along with the Viral RNA extraction kit. This carrier RNA plays a crucial role in RNA extraction as it enhances the overall quantity and quality of the eluted RNA. The kits are highly sensitive and provide the end-user with accurate and reliable results. Most of these kits are also compatible with top-notch automated nucleic acid isolation machines available in the market. The kits are compatible with manual isolation of RNA being done at designated COVID-19 testing laboratories.
Viral RNA extraction is an essential aspect of Coronavirus diagnostics. Without good quality and quantity of RNA, Real-Time PCR diagnostics for testing Coronavirus is not feasible. Viral RNA Extraction kits should be used for RNA isolation and escalate the process of COVID-19 testing. More tests will lead to more isolation of patients, which will contain the spread of the virus.
Till the vaccine's availability, Stay safe, Wear a mask and follow the norms of social distancing.