#enteric pathogens

[tmmediapharma]

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If you are looking for SS Agar, or Salmonella-Shigella Agar manufactured by TM Media, stands as a crucial medium in microbiology designed for the isolation and differentiation of enteric pathogens, specifically Salmonella and Shigella species. Composed of a carefully balanced formulation, SS Agar incorporates selective agents and indicators to facilitate the growth and identification of these bacterial strains. The key components of SS Agar include bile salts, brilliant green dye, and a mix of carbohydrates like lactose and sucrose. Bile salts act as selective agents, suppressing the growth of gram-positive bacteria and many gram-negative organisms, except for Salmonella and Shigella.

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[mbimphblog]

PHYTOCHEMICAL SCREENING AND ANTIBACTERIAL PROPERTIES OF Punica granatum EXTRACTS AGAINST GASTROINTESTINAL INFECTION AN In-vitro STUDY

Background: Punica granatum Linn is a bearing fruit shrub, belonging to the family Lythraceae. Every part of this plant is useful for humans such as seeds and fibers endow with vitamins-C, K and folate. Oil acids of punica, palmitic, stearic and oleic acid present in the seeds. Pomegranate is generally used as a conventional medication in India for the cure of enteric bacterial pathogens.

Aim: To study the phytochemicals and antibacterial activities of aqueous, alcoholic and chloroform crude extracts of P. granatum peel against some enteric pathogens.

Materials and Methods: The isolates were examined by Foldscope microscopy and biochemical tests. Phyto-chemical study analyzed the existence of alkaloids, flavonoids, phenols, saponins, terpenoids, steroids, tannins, reducing sugars, carbohydrates, and amino acids. Vancomycin, tetracycline, chloramphenicol, gentamycin, and co-trimoxazole are the antibiotic disc used in the disc diffusion assay.

Results: A multidrug-resistant pattern was observed in the isolated pathogens. As reported by natural medicine P. granatum is used for the treatment of enteric pathogens. Antimicrobial activity was performed by the standard procedure (Kirby-Bauer’s – agar well diffusion method) using pomegranate extracts (aqueous, alcoholic and chloroform) against the enteric pathogens. Peel crude extracts of P. granatum were showed various degrees of antibacterial activities with the maximum mean inhibition zones reaches 36±1.0 mm in diameter for methicillin-resistant Staphylococcus aureus (MRSA) with ethanolic extract. The highest zone of clearance was found in ethanolic extract followed by aqueous extract, but only the lowest zone was in chloroform extract. Most significant inhibition was observed against Staphylococcus aureus, Vibrio cholerae, Pseudomonas aeruginosa, Proteus vulgaris, E. coli, Salmonella typhi, Shigella dysentriae followed by Klebsiella sp.

Conclusion: The end result attained that the peel extracts of Punica granatum have impressive pharmaceutical bioactive compounds and antibacterial property. It is used as natural medicine by itself to treat gastrointestinal disorders.

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[resci]

"Recent gut microbial surveys have indicated dramatic shifts in gut microbial population structure from infants to young adults to the elders. An understanding of the evolution of these factors and their interactions (e.g., how does gut microbiota modulate the "inflamm-aging" process or vice versa) through the human life "cycle" will be important in better addressing and controlling these enteric infections and their consequences for both quality and quantity of life (often assessed as disability adjusted life-years or "DALYs")."