#escrt

[no-pida-cafe-blog]

(via no-pida-cafe)

Fragmentos.

[designinbiology]

Snf7, a component of the ESCRT-III membrane fission complex, spontaneously polymerizes into spiral springs that promote membrane deformation when they relax from compression. The cover art represents the various shapes generated by Snf7 polymers on a lipid bilayer. Snf7 has a preferential curvature, but steric constraints lead to stress zones with over- or under-curvature, indicated in light blue.

by Cell 

Aurélien Roux Lab (Universtity of Geneva)

brought to you by GSS

[javlington-blog]

Kalinowska et al. 2015 - Proceedings of the National Academy of Sciences

ALIX can directly interact with AMSH3

alix null mutants show similar phenotypes to amsh3

ALIX Is required for vacuole biogenesis

AMSH3 shows colocalization with late endosomal markers ARA6/7

AMSH3 and ALIX colocalize on late endosomes

ALIX binds ubiquitin and is involved in ubiquitin-dependent protein degradation

ALIX Is important for localization of AMSH3 on ARA7-labeled vesicles

This related study published a couple of days later describes that ALIX interacts with ESCRT-III component SNF7 on late endosomes/MVBs. The authors also independently found ALIX involvement in vacuole biogenesis, and interestingly also in vacuolar trafficking of the RLK BRI1, and plasma membrane-localized phosphate transporter PHT1: ESCRT-III-associated protein ALIX mediates high affinity phosphate transporter trafficking to maintain phosphate homeostasis in Arabidopsis - Cardona-López et al. 2015 The Plant Cell

More about AMSH3/ESCRT-III:

The Arabidopsis deubiquitinating enzyme AMSH3 interacts with ESCRT-III subunits and regulates their localization - Katsiarimpa et al. 2011 The Plant Cell

The ESCRT-III-interacting deubiquitinating enzyme AMSH3 is essential for degradation of ubiquitinated membrane proteins in Arabidopsis thaliana - Katsiarimpa et al. 2014 Plant & Cell Physiology

Proper AMSH3 interaction with ESCRT-III components and K63-linked deubiquitinase activity is required for defense - Schulz-Larsen et al. 2014 XVI IS-MPMI, Rhodes, Greece

Arabidopsis Endosomal Sorting Complex Required for Transport III (ESCRT-III) regulates internal vesicle formation of prevacuolar compartment and is required for plant development - Cai et al. 2014 Plant Physiology

More about MVB biogenesis and maturation:

Biogenesis of plant prevacuolar multivesicular bodies - Cui et al. 2016 Molecular Plant

Phosphatidylinositol 3-phosphate 5-kinase, FAB1/PIKfyve mediates endosome maturation to establish endosome-cortical microtubule interaction in Arabidopsis - Hirano et al. 2015 Plant Physiology

FYVE1 is essential for vacuole biogenesis and intracellular trafficking in Arabidopsis - Kolb et al. 2015 Plant Physiology

A unique plant ESCRT component, FREE1, regulates multivesicular body protein sorting and plant growth - Gao et al. 2014 Current Biology

A critical role of LIP5, a positive regulator of multivesicular body biogenesis, in plant responses to heat and salt stresses - Wang et al. 2015 Plant Physiology

Arabidopsis LIP5, a positive regulator of multivesicular body biogenesis, is a critical target of pathogen-responsive MAPK cascade in plant basal defense - Wang et al. 2014 PLoS Pathogens

[biolegend]

We've launched a new page detailing information about exosomes and their functions. Check it out at: http://www.biolegend.com/exosomes

[legalizad0]

Quando criança achava que ninguém envelhecia. Mas hoje quem envelhece sou eu.

Hugo Santos.

[legalizad0]

Veja como o tempo voa, demora para aterrissar.

Sem graça.