Jan 18, 2022
Mitochondrial Protein Mass Spectrometry Identification
The basic principle of mitochondrial protein mass spectrometry identification is to digest proteins into peptide mixtures by protease, ionize them by soft ionization methods such as MALDI-TOF or ESI-MS, and then separate peptide ions with a specific mass / nuclear ratio by mass analyzer. The protein is identified by comparing the actual spectrum with the theoretically first-and second-order mass spectra produced by protease digestion.