Dr. Elinne Becket, a microbiologist from Cal State University, is in the middle of one of those Fridge Experiments that happens to us all - except in this case, she is uniquely placed to unravel the science down to the microbial level.
While cleaning out her fridge, Dr. Becket found that a tub of family-recipe beef vegetable soup had turned bright blue. “Ok I'm outing myself here,” she tweeted, “but there was forgotten beef soup in our fridge we just cleaned it out and it was BLUE?!?!? Wtf contam would make it blue??? Like BRIGHT blue!! Even w/ all my years in micro I'm not handling this well.“
Read on for a breathless and ongoing saga of Soup and Science, and the wonderful international community that is Academic Twitter.
Academic Twitter quickly reminded her of her Responsibilities to Scientific Inquiry. (Cue the chanting from around the world of “CLONE THE SOUP! CLONE THE SOUP!”)
“I can’t believe y’all talked me into going back into the trash.” she tweeted in response, over a photo of a puddle of beautiful Mediterranean-sea blue soup in the trash bin, with bits of veg and noodles arising from the depths.
Scientists being scientists, Dr. Becket agreed to take a sample and send it to colleagues for cloning and microbial analysis.This involved getting arms-deep into the trash bin of Old Soup. “I’m never forviging @ATinyGreenCell (genomic biologist Sebastian Cocioba) for this.” Dr. Becket tweeted, with a photo of a properly dipped and snipped and VERY blue q-tip in a small clear plastic tub.
Diving into decomposing soup was not the only hazard. She writes: “My mom (who made the soup for my birthday) came across this thread and now 1) I have to answer for letting her soup spoil and 2) she's worried @ATinyGreenCell will figure out her secret recipe.“
Dr. Becket and Sebastian were able to culture the Blue Goo!
Becket posted a photo of three petri plates of streaked beef bouillon agar at 72 hours incubation, at 37C, room temp and 4C. She writes: “Left the plates where they were for another 2 days, except the 37°C one was brought to RT, which then grew white stuff over the yellow stuff and stinks to high heaven. RT looked the same. 4°C had impressive growth. Restreaked them all onto TECH agar, awaiting results!”
Sebastian, from his lab, tweeted a photo of three more covered petri dishes, with early results: “Great progress on isolating the glowy microbe from our #BlueSoup! It's so fluorescent the streak is GREEN. Still needs another restreak as it seems there is a straggler but should clear up in the next plate. Exciting!”
Then yesterday, Sebastian tweeted out an updated photo of his plates under daylight and blacklight. “Whatever grew on the #BlueSoup colony plates overnight glows under UV, but only on King's Agar B! That particular media is used to tease out fluorescein expression in pseudomonads. What are the chances that the same cell line expresses fluorescent AND blue pigments?“
“Looking closer, there definitely is a handful of different microbes showing distinct phenotypes. Could be that the blue producer and the fluorescent microbes are totally different microbes!”
At which point, Professor Cynthia Whitchurch of Norwich, England, responded: “Consistent with P. fluorescens being at least part of the #BlueSoup community. The fluorescence is due to production of the siderophore pyoverdine which is up-regulated when iron availability is limited. P. aeruginosa produced this too but my guess is you have blue Pf.”
And Australian agricultural researcher @WAJWebster helpfully tweeted a petri dish of ALL KINDS of colourful bacterial colonies from white to yellow to orange to stark black, with a cheerful: “You need bact-o--colours? I got you, fam.”
The best part is that as of today, March 9, 2023, THE BLUE SOUP MYSTERY CONTINUES. WE ARE WATCHING SCIENCE HAPPENING!
A paper is being written. And Dr. Becket’s mum is getting an author credit as the proprietary owner of the #BlueSoup recipe.
Dr. Becket’s Twitter is here: https://twitter.com/bielleogy
Sebastian Cocioba’s Twitter is here: https://twitter.com/ATinyGreenCell
Fun IFLS story is here: https://www.iflscience.com/microbiologist-investigates-after-her-beef-soup-turned-blue-in-the-freezer-67894?fbclid=IwAR0H27KqVZhzzrosnjzzKkxuKASZ-0L0Lt6hGwCRDJK8xvFbbSlyS4JvwlM
