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Microscopic examination of Giemsa-stained blood slides has generally been considered the gold standard in disease diagnostics. Microscopy, however, relies heavily on the knowledge, level of skill, and judgement of the user, which could affect its sensitivity and specificity. In low-resource settings such as remote health outposts, it is difficult to find, train, and support proficient microscopists, especially those capable of detecting early infection.
If you have been following IV Lab for a while, you likely know that we have been working on new diagnostics technologies for some time. One of the diagnostic platforms our scientsts and engineers are developing is an automated optical diagnostic microscope. The Autoscope aims to be a low-cost, portable, fast, automated miscroscope that can detect stained parasites using image-processing algorithms.
Slide preparation using Giemsa stain for research in malaria diagnostics at Intellectual Ventures Laboratory. Giemsa smear microscopy was developed over a century ago and is still widely used to diagnose malaria. Learn more at http://intellectualventureslab.com
Pic I took to some CCD-34Lu cells, (Human fibroblast), coloured with Giemsa. You can notice some of them in mitosis!
A photomicrograph of the histological changes caused by ''Myxobolus cerebralis'' infection (Whirling disease) of salmonid cartilage, stained with Giemsa.
From the collected images of Dr. Thomas L. Wellborn, Jr. (Wikipedia)
Hari Kedua \(^o^)/
Mari berbincang tentang hari ini yang semakin melelahkan saja. hari ini agak terlambat, karena harus berjalan kaki menuju tempat kerja. kerja ?! oups! maksudnya tempat magang, red. Kali ini masuk lewat pintu tengah, mencari suasana baru *alibi, bilang aja males lewat emergency soalnya ada ..........tiiiiiitsensor*
Memakai jas lab, masuk ruang sampling, dan siap tempur! bersama om vampir mengarungi indahnya sedot menyedot darah orang.. duduk di depan layar komputer, entry data ke KMS *lagi*, sepertinya semakin mahir, hhaa, i like this.
Pasien hari ini tidak seramai kemarin, kali ini 66org :) jadi, ada banyak cara untuk mencuri-curi waktu menuju pantry (kebetulan hari ini saya sedang tidak berpuasa). Berbincang, bergosip dengan perawat tentang dokter muda 'itu', menikmati milo hangat bersama beberapa petugas X-ray.
TO-DO-list hari ini :
07.00-11.00 menjadi asisten om vampir di ruang sampling.
11.00-11.30 menyelesaikan deadline hasil pemeriksaan ke dalam KMS.
i did a mistake : lupa melingkari nomor urut pasien yang melakukan pemeriksaan CBC (cell blood count) --> fatal red ! ujung-ujungnya diomelin mas rin, kerja ekstra.
11.30-13.00 makan siang
13.00-14.00 Kimia Klinik - URINE
Alat ( urine chemistry analyzer ) : Meditron Junior II ( http://www.medcompare.com/details/32333/Miditron-Junior-II.html )
Pemeriksaan Carik Celup / Test Strips
14.00-14.30 melamun + terkantuk-kantuk ( baca SOP )
14.30-15.00 Bertemu Ibu Ing *felt so nervous
skipshit. jam kerja ditambah. harusnya sampe jam 15.00, katanya sekarang sampe jam 16.30, mulai hari ini sampai 3minggu ke depan + bikin laporan tiap hari tentang apa yang dipelajari :(
15.00-15.30 Pewarnaan Giemsa
Pada beberapa slide Apus darah tipis dan tebal yang belum dicat. Saya melakukan pewarnaan dengan perbandingan reagen Giemsa : Air = 1 : 4 ... didiamkan 10-15 menit, lalu dikeringkan. sebelumnya, Fiksasi apus darah tipis dengan metanol, metanolnya abis jadi pake alkohol. agar eritrosit lisis. dan kedua apus darah tersebut, dalam satu slide.
Apus Darah Tipis : untuk menentukan jenis malaria
Apus Darah Tebal : hitung jumlah. jumlah parasit / 200 leukosit (hanya pada Plasmodium falciparum)
berikut contoh apus tebal (gambar atas) dan apus tipis (bawah) :
15.30-16.30 menerawang malaria di mikroskop *yg uda disediain mas rindra, hhe*
ok. done for today.
without raining.
happier.
:)