Wilt disease is one of the problems in maize production caused by fungi and bacteria. Tanah Laut Regency, the center of maize production in South Kalimantan, also experienced this disease. Accurate identification of pathogens that cause wilt disease in maize in South Kalimantan, including in Tanah Laut District, has not been reported. Therefore, this study was conducted to identify the cause of wilt disease in maize. This research was carried out at the maize center in Tanah Laut Regency, South Kalimantan; the Phytopathology Laboratory, Faculty of Agriculture, Lambung Mangkurat University, Banjarbaru; and the Class I Agricultural Quarantine Laboratory, Banjarmasin. Collection and selection of bacteria isolated from sample plants were carried out to obtain pathogenic bacteria that cause wilt disease in maize and then tested biochemically and biomolecular. This study found 10 isolates of pathogenic bacteria that attack maize crops in Tanah Laut Regency. The ten bacteria belong to 4 genera, namely Pantoea, Erwinia, Dickeya, and Pectobacteria. Biomolecular tests found one isolate suspected to be Pantoea stewartii. published by the International Journal of Biosciences | IJB