The study focused on evaluating the results of in vitro fertilization from very low sperm count and sperm from testicular microsurgery in NOA patients from different ages. Study subjects were divided into 2 groups: under or above 35 years old group; and each group was divided into 2 subgroups: T-ICSI and E-ICSI. The evaluation criteria mainly focused on the fertilization rates, the good embryo rates and the clinical pregnancy rates. The results showed that, in the under 35 years old group, the fertilization rates in the T-ICSI group were significantly higher than the E-ICSI group. However, the third day good embryo rates, the blastocyst formation and the good blastocyst rates in the both T-ICSI group and the E- CSI group were similar. In the above 35 years old group, the fertilization rates, the third day good embryo and the good blastocyst rates in the T-ICSI group were significantly higher than E-ICSI group The percentage of live births in the T-ICSI group was higher than the E-ICSI group, but there was no statistical difference. Especially, the weight of children born from the group using sperm from microTESE was heavier than that of children born from the group of sperm from Cryptozoospermia. The results of the study showed that sperm extraction from testicular microsurgery improved embryology results in the above 35 years old of males with non-obstructive azoospermia. Regarding clinical treatment results, the group using sperm from testicular microsurgery tended to be higher than the group using sperm from ejaculate samples.
