#mohsprocedure

[thatmohstech]

Basal cell carcinoma with H&E and toluidine blue

[thatmohstech]

Infiltrative basal cell carcinoma in a first stage Mohs layer stained with H&E and toluidine blue at 2X

Notice the epidermal layer in the top right corner

[thatmohstech]

Basal cell carcinoma, nice and obvious!

This is a first stage Mohs layer

[thatmohstech]

Piece of a right helix with cartilage stained with toluidine blue, 8 microns 4x objective

I've had the best luck using SlideMate Plus Slides particularly in the Mohs lab both for cartilage and for IHC. I've tried other brands of charged slides that didn't work as well.

When I have cartilage, I put the slides on a hot plate on its lowest setting for 1-2 minutes (not much more!!! Or else the Oct bakes), then put them in 100% for 1 minute, a quick dip in water, and then I continue the H&E stain from the hematoxylin (or go into T. Blue)

[thatmohstech]

Heart shaped first layer lol

Valentine's day themed mohs surgery

[thatmohstech]

A section of cartilage (second stage Mohs layer) stained with both toluidine blue and hematoxylin and eosin

[thatmohstech]

Nodular basal cell carcinoma in a first stage Mohs layer

[thatmohstech]

Nodular basal cell carcinoma stained with toluidine blue