The present study focuses to examine the meristic and morphometric variations of Ompok pabo from three populations namely Bergobindapur baor (BB), Bhairab River (BhR) and Kopotakho River (KR) in Bangladeshi freshwaters using the landmark-based truss network analysis. Truss protocol used in the present study based on seven general morphometrics and eight landmarks points constructed by interconnecting them to form total 16 truss measurements. Meristic counts were compared among three populations and no significant differences were observed in non-parametric Kruskal Wallis test. Univariate statistics (ANOVA) showed 5 [standard length (SL), post-orbital length (PsOL), maximum body depth (MBD), length of left barbel (LLB) and length of right barbel (LRB)] of seven morphometrics measurement and 8 (2-3, 3-4, 6-7, 2-7, 2-6, 3-6, 3-5, 2-8) of the 16 truss measurements significantly differed to varying degrees (p