Simple Green Synthesis of Dextrose Reduced Silver nanoparticles and their Antimicrobial activity
We herein report the controlled synthesis of highly monodispersed, water soluble, stable, smaller sized, gelatin capped-silver nanoparticles (Ag-NPs), via an eco-friendly, completely green method. The method involves the use of silver nitrate, starch and dextrose as the silver precursor, stabilizing agent and reducing agent respectively in aqueous solution without the use of any accelerator. By varying the reaction time, we monitored the optical and structural properties of the colloidal Ag-NPs. The nanoparticles were characterized using UV-vis absorption spectroscopy, Fourier transform infra-red spectroscopy (FTIR), transmission electron spectroscopy (TEM), high resolution electron transmission microscopy (HRTEM), selected area electron diffraction (SAED) and energy dispersive spectroscopy (EDS). The absorption maxima of the as-synthesised materials at different reaction time showed characteristic silver surface plasmon resonance (SPR) peak. The TEM images showed well-defined monodispersed quasi-spherical particles with particle size in the range 3.5-9 nm at 24 h reaction time while the presence of the lattice fringe in the HRTEM micrograph confirmed the high crystallinity of the as-synthesized material with face centered cubic (fcc) structure. The antimicrobial properties of the as prepared Ag nanoparticles where tested using Escherichia coli, Staphylococcus hominis and pseudo aeruginosa. This synthesis offers a cleaner and greener method for the controlled synthesis of Ag-NPs without the use of any hazardous complexant or accelerator.
Keywords:silver nanoparticles, gelatin, dextrose, green synthesis, optical spectroscopy, electron microscopy.
1Sneha Mohan, 2Oluwatobi.S Oluwafemi, 3Sandile P. Songca, 1,4Nandakumar Kalarikkal, 1,5Sabu Thomas
1. Centre for Nanoscience and Nanotechnology, Mahatma Gandhi University, Kottayam, Kerala-
686560, India. ([email protected])
2. Department of Chemistry and Chemical Technology, Walter Sisulu University, Mthatha
Campus. Private bag XI, Mthatha 5117, Eastern Cape, South Africa.
3. Faculty of Science, Engineering and Technology, Walter Sisulu University, PO Box 19712, Tecoma, East London.
4. School of Pure and Applied Physics, Mahatma Gandhi University, Kottayam, Kerala-686560,
India.
5. School of Chemical Sciences, Mahatma Gandhi University, Kottayam, Kerala-686560, India