#paraffinembedding

[labtronequipments]

Tissue Embedding System LTES-A10 - Embedding Machine

Labtron Tissue Embedding System uses 220 V/50 Hz power: 650 VA for main/thermal units and 200 VA for the cryo unit. The main console spans ambient to 80°C in 1°C steps, while the thermal console offers adjustable heating and a large paraffin tray. The cryo console provides adjustable cooling with precise control for tissue processing.

[kirloskar]

Silent Symphony of Tissue Processing with HistoTek

The HistoTek Vacuum Infiltration Processor introduces a new beat to the world of histopathology because it provides uniform tissue infiltration with an unparalleled degree of consistency. HistoTek is engineered to facilitate seamless paraffin embedding and to manage delicate reagents during the processing of fine tissue samples. It has an easy to use design that reduces the use of manual work and maintains the structural integrity of specimens. Whether it is biopsy analysis or large tissue blocks, the repetitions of the cycles guarantee the reproducibility, accuracy, and safety. The efficiency of the HistoTek enables the technicians to work on the quality of the diagnostic use, but not on the complexities of the operations, and reshape the standards of modern histotechnology with the use of intelligent automation and reliable functioning.

[immunostainingus-blog]

Paraffin Embedding Overview - Immunostaining.info

Paraffin embedding is one of the standard procedures used in preparing slender sections of biological substance for histological assessment by light microscopy. There are major steps that are involved in paraffin embedding includes fixation, dehydration, transparentizing, immersion and embedding.

The tissues samples collected are usually submitted to regular pathologic analysis and then preceded through fixation and paraffin embedding. Fixation procedure is used to cross-link the antigens to well-built macromolecules, thereby immobilizing them in tissue.

Furthermore, paraffin embedding may have an effect on the immunoreactivity. For predictable histology, fixation of tissue, followed by paraffin embedding is the most widely-used method. It is criterion in the majority of histology laboratories, and is appropriate for assessing lymphoid tissue.

Here are the 5 steps described that are involved in paraffin embedding:

Fixation

The main purpose of this process is to keep cell sharp and tissue in proper shape to prevent putridness, postmortem autolysis, endogenic and exogenic enzyme activity. Maintain the cell structure as well as position by preventing antigen diffusion. It gives color to clarify tissues by diverse affinity to coloring agent.

Dehydration

In this step the water is completely removed and creates a situation for the next step and hardens the tissue of curiosity. The dehydrating agents Ethanol and Acetone are completely miscible with water and can be arranged in different volumetric ratios.

Transparentizing

The tissue of interest necessitates a transparentizing move for the reason that the dehydrating agent used in the above step is immiscible with the paraffin. The adding up of transparent reagent helps paraffin soak up into the tissue.

Immersion

When the transparentizing process is completed, the tissue is then immersed in molten paraffin wax so that it takes up the wax-substituting transparent source. Based upon the melting temperature of paraffin wax, immersion should be implemented in between 54℃ to 64℃.

Embedding

In embedding the process is carried of treating the tissue in a paraffin box so that the paraffin wax cools down plus solidifies. Once cooled, the tissue will be ready for sectioning and appropriate for storage.

About Boster Bio

As the leading Flow Cytometry Technical Resource Centers, Boster Bio was founded by Steven Xia, histologist, in the year 1993. The business currently provides hybridoma generation check to product custom monoclonal antibodies. For more information on Paraffin Embedded Tissue, please visit https://immunostaining.info.

Contact Address

Boster Biological Technology

3942 Valley Ave

Pleasanton, CA 94566, USA

Email:[email protected]

Phone: [phone]

[immunostainingus-blog]

Paraffin Embedded Tissue

Paraffin Embedding involves the steps fixation, dehydration, transparentizing, immersion and embedding. Paraffin Embedded Tissue is sliced by a rotary microtome to give a thickness of 2-7 μm.

[immunostaining12-blog]

IHC Tissue Processing for Paraffin Embedding | Immunostaining

Paraffin embedding is the process of treating in the paraffin box so the paraffin wax cools down solidifies. An alternative approach, suitable for thin or porous tissues, is to perform IHC on fresh tissues and then post – fix and embedded the tissues in paraffin.

[immunostaining12-blog]

Tissue Fixation and Paraffin embedding protocol | Immunostaining

When generating paraffin  embedded tissue samples, the tissue must be fixed before embedding in paraffin. Fixation is achieved by perfusion or immersion immediately following dissection.  This process typically takes 4-24 hours.

[immunostaining12-blog]

Five major steps are involved in paraffin embedding: fixation, dehydration, transparentizing, immersion and embedding. Learn several different methods for tissue embedding.