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WB Essential guidance| After returning to school you must know the product portfolio of WB protein analysis
Following the previous issue of “WB Essential guidance | WB Sample Pre-Processing Product Portfolio Scheme that You Must Know After Back to School”, the little friends have a deep understanding of the sample pre-processing after learning. The editor here also received the ardent new and old customers of Abbkine eager for Abbkine products. Therefore, the second part of WB dry goods series: After returning to school, you must know the product portfolio plan after WB transfer film, which came into being, and continue to answer questions for everyone.
In the last issue, we talked about protein quantification products. Everyone may be curious, how should protein quantification products be selected? Next announced for everyone:
The most popular methods for protein quantification are BCA and Bradford:
BCA method: tolerant to detergents: SDS, TritonX-100, Tween-20, etc., but not to reducing agents, if the sample contains detergents without reducing agents, this method can be used (recommended KTD3001)
Bradford method: can tolerate the chemicals in most samples, not high concentration detergent, if the sample contains high concentration detergent, this method can be used (recommended KTD3002)
After protein quantification, doing protein analysis is naturally the most concern of WB experimenters.
The first step is to load the sample. In the sample loading process, in addition to the sample order that you cannot forget, the most important thing is the protein marker. In order to clearly indicate the molecular weight of the protein, some senior WB operators will choose the point 2-3 pre-stained protein wells to avoid experimental uncertainty and improve the clarity of instructions. Here we recommend two color pre-stained protein markers:
From the experimental results, the BMM3001 and BMM3002 markers have bright colors, and the sharpness and contrast are very high. The reference value of the indication result is very high.
After a long and lengthy step of running rubber and transferring membrane, it finally entered the stage of WB immunization. At this time, how to correctly select the internal reference antibody will become an important step in the WB experiment. The following dry goods are here again-the three magic weapons for the correct internal reference selection:
Species source: mammalian tissue or cell samples, usually choose β-actin, β-tubulin, GAPDH, etc., plant experiment samples use plant actin, Rubisco, etc.
Protein molecular weight: The molecular weight of the target protein should be considered. Generally, the molecular weight difference between the target protein and the internal reference protein should be more than 5KD; for high molecular weight proteins over 100 kDa, Vinculin focal adhesion protein is recommended as the internal reference antibody.
Protein expression site: According to the different protein expression positions, more suitable antibodies can be selected. Commonly used extensive β-actin, β-tubulin, GAPDH; detection of nuclear Lamin A, Histone H3, PCNA, detection of cell membrane Na, K ATPase, etc.
In addition to free antibody, Abbkine’s internal reference antibody also has a straight-labeled antibody conjugated with a label. If you think it is not enough, you can also contact our Abbine technical experts ([email protected]) for in-depth communication. We will help you find the most suitable internal control antibody for your experiment.
In addition to internal reference antibodies, the selection of target protein antibodies is the finishing touch of our WB experiment. At this point, you may consider: how to select antibodies with strong specificity, high sensitivity, and more literature support? The first choice is Abbkine’s first antibody. After hearing the name, we know that our antibody has been carefully tested by Abbkine scientists on the functional properties of the antibody. So the customers who used it are full of praise. Do not believe? You can also try.
Of course, Abbkine’s common monoclonal antibodies and polyclonal antibodies are also effective and cost-effective. Used children’s shoes are said to be good. If the customer’s sample is a recombinant protein with a protein tag, we can also provide the corresponding labeled antibody and its direct-labeled antibody to meet the needs of customers with a variety of experiments.
Product NO Product Name A02010 Anti-DDDDK Tag Mouse Monoclonal Antibody (1B10) A02020 Anti-GFP Tag Mouse Monoclonal Antibody (3D3) A02030 Anti-GST Tag Mouse Monoclonal Antibody (2A8) A02040 Anti-HA Tag Mouse Monoclonal Antibody (4F6) A02050 Anti-His Tag Mouse Monoclonal Antibody (5C3) A02060 Anti-Myc Tag Mouse Monoclonal Antibody (2D5)
Of course, many customers are interested in the coupling kits independently developed by Abbkine. Our company also has carefully created the LIKINE series of kits for you. The self-conjugated antibodies will also be very fulfilling to use.AbFluor™ dyes are a series of highly water-soluble fluorescent dyes spanning full UV-visible and near-infrared spectrum for labeling biomolecules, especially proteins and nucleic acids. AbFluor™ dyes have much better labeling performance than other fluorescent labeling dyes such as FITC, TRITC, Cy® dyes, Alexa Fluor® and Dylight® labeling dyes.
At the end of the experiment, of course, the use of color developing solution, Abckine’s ECL color developing solution can reach the pico level and fly level. This sensitivity is very impressive. SuperLumia high-sensitivity and ultra-sensitive ECL products have extremely high sensitivity, and require less samples and antibodies; the luminous signal is strong and lasts for a long time.
Product No Product Name K22020 SuperLumia ECL HRP Substrate Kit K22030 SuperLumia ECL Plus HRP Substrate Kit
In order to provide readers with a platform for learning biological knowledge, Abbkine’s technical experts have worked hard to sort out the dry goods of various high-end biological experiments. Later, they will be published in the WeChat public account, and readers who love science will continue to pay attention to the Abbkine public account. .
Abbkine specializes in the fields of proteinology and cytology, and is committed to innovating and developing various antibodies, proteins, analytical reagents and kits, with a view to becoming a key promoter in the fields of life science research and development, drug research and development. We provide you with favorite products of protein and immunological research users, from basic immunological products, such as protein extraction and quantification, to internal reference labeling antibodies, primary antibodies and secondary antibodies of immunological experiments, etc.; favorite products of cell research users, from Dyes and kits for detecting the state of cells, kits for extracting organelles, staining and tracking of cell substructures and cell metabolism detection products, to cytokine and protein detection kits for cell culture, only to help your research career !
About Abbkine
Our position: to serve global users of cell and protein research, to provide users with economical and technical product solutions in the form of application processes and product portfolios.
Our mission: to stimulate our inner creativity, provide competitive biomedical products and services, and continue to create maximum value for customers.
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