Script Draft 2... Whitney
Title: Experimenting in the lab
[Title, music, cuts of me walking through the lab, putting on gloves, safety glasses, lab coat]
Welcome to The Lab! Let’s take a look at the materials for our experiment. [Camera pans around to lab bench]
[YouTube link on the bottle of H2O2] [Pick up the bottle of H2O2]
This is hydrogen peroxide. You may recognize it from your first aid kit at home. If I left this bottle sitting here, the hydrogen peroxide would eventually break down into water and oxygen gas. This process - when you have chemical compounds that break apart to form smaller, simpler substances - is called decomposition. Since this is normally a slow process, it wouldn’t be very exciting for us to watch, right? We need to add something that will speed it up – a catalyst.
[Pan back around to lab bench and have YouTube link on the packet of yeast]
Here we go - yeast! Yeast produce an enzyme called catalase. Actually, your body has this enzyme, too! Organisms make this enzyme to protect against hydrogen peroxide damaging cells. This catalyst is the special sauce in our recipe. Catalase speeds up the decomposition of hydrogen peroxide into water and oxygen gas. But how does a catalyst speed it up? Picture this - I want to roll this ball down the hill to my friend at the bottom, but there is a big, tall rock in the way. I could get the ball over the big rock, but it would take a lot of time and energy. I look around and find a spot with smaller rocks that would be easier to get the ball over and down to my friend. For hydrogen peroxide under normal conditions, there is essentially a big rock in the way – so the breakdown is slow because it takes a larger amount of energy to get over the barrier. But with catalase, the rocks in the way are smaller. This different pathway doesn’t take as much energy so the reaction happens faster. We need to add one more thing to our experiment!
[Pan back around to lab bench and have YouTube link on the bottle of soap]
We are going to add some soap! The reaction is going to produce a lot of oxygen gas. This would normally be released out into the air, but by adding soap, the oxygen will get trapped in the soap bubbles instead. This foam is going to help us see what’s happening during the experiment.
We’re ready for the experiment! [Do one elephant toothpaste reaction] But wait… we’re not done yet. What would happen if we changed the recipe? Now that we know all the pieces of the reaction, we can play around with the recipe to try to better understand the factors that influence it. This is what a scientist does. So what are we playing around with when we change the recipe? Variables! There are three important variables to an experiment. Something that you change, or manipulate, is called the independent variable. After you change something, you then wait to see what happens to something else – the dependent variable. Everything else should stay the same because we want to be sure that the independent variable is causing the change in the dependent variable. Which variable should we change in our experiment?
[Hold up H2O2 and yeast - Viewer clicks to choose]
[H2O2 script]
Let’s make a hypothesis! What do you think will happen if you change amount of H2O2? [Viewer chooses hypothesis]
If I increase the concentration of H2O2, then ______________.
[Focus group on Monday: get hypotheses from the kids]
[Experiment is the same for each hypothesis – just a different ending for the revisit]
Here we have four beakers filled hydrogen peroxide mixed with water. Each beaker has a different strength, or amount, of hydrogen peroxide: 5%, 10%, 15%, 30%. We only want to observe how the amount of hydrogen peroxide affects how much foam is made during the reaction. We don’t want to change anything else so we’ll use the same amount of yeast and soap each time. Let’s do the experiment and test your hypothesis.
[Camera frames H2O2 vials on bench - viewer chooses H2O2 concentrations to test] [Spontaneous dialogue during experiment]
[Next segment depends on hypothesis]
You predicted that _______.
[Correct] What we saw supports your hypothesis! But, remember, it’s not about being right or wrong, it’s about what we learned in the process.
[Incorrect] That’s not what we saw during the experiment. But, remember, it’s not about being right or wrong, it’s about what we learned in the process.
As we increased the H2O2 concentration, we saw more foam formation. But why? Let’s look at the chemical reaction: 2 H2O2 -> 2 H2O + O2. H2O2 is a reactant and water and oxygen are products. When 2 molecules of H2O2 decompose, one molecule of oxygen is formed. So by increasing the amount of H2O2, the amount of oxygen formed through the catalyzed decomposition reaction also increases. More oxygen results in more foam!
You just learned about a cool reaction, but more importantly, today, you were a scientist! Scientists don’t have a recipe book to follow, so this process of experimentation is how scientists approach solving problems. Just like the scientists that study new materials for solar cells to power your home, or research a cure for cancer, you can be a scientist by following the scientific method.
Ask questions, make predictions, experiment, observe and measure, analyze data, formulate conclusions, and always ask new questions.
Now, go experiment!
[Link to click to experiment with the other variable.]
[Yeast script]
Let’s make a hypothesis! What do you think will happen if you change the amount of yeast?
[Viewer chooses hypothesis]
If I increase the amount of yeast, then ______________.
[Focus group on Monday: get hypotheses from the kids]
[Experiment is the same for each hypothesis – just a different ending for the revisit]
Here we have four beakers with different amounts of yeast: ½ packet, 1 packet, 2 packets, 3 packets. We only want to observe how the amount of yeast affects how fast the foam is made. We don’t want to change anything else so we’ll use the same amount of hydrogen peroxide and soap each time. Let’s do the experiment and test your hypothesis.
[Camera frames yeast on bench - viewer chooses beakers with yeast to test] [Spontaneous dialogue during experiment]
[Next segment depends on hypothesis]
You predicted that _______.
[Correct] What we saw supports your hypothesis! But, remember, it’s not about being right or wrong, it’s about what we learned in the process.
[Incorrect] That’s not what we saw during the experiment. But, remember, it’s not about being right or wrong, it’s about what we learned in the process.
As we increased the amount of yeast, we saw the foam was formed faster. But why? In each experiment, the amount of hydrogen peroxide was the same. Chemical reactions have fixed stoichiometry. This means that every two molecules of H2O2 breaks down into two molecules of water and one molecule of oxygen. This doesn’t change, even with a catalyst, so each reaction we did produces the same amount of oxygen. But, this is a catalyzed reaction. When we increase the amount of yeast present, there is more catalase enzyme hanging around to break down the H2O2. So when the extra catalase gets through the stock of H2O2 faster, oxygen is also released (and foam formed) faster.
You just learned about a cool reaction, but more importantly, today, you were a scientist! Scientists don’t have a recipe book to follow, so this process of experimentation is how scientists approach solving problems. Just like the scientists that study new materials for solar cells to power your home, or research a cure for cancer, you can be a scientist by following the scientific method.
Ask questions, make predictions, experiment/observe/measure, analyze data, formulate conclusions, and ask new questions.
Now, go experiment!
[Link to click to experiment with the other variable.]














