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TLC HPTLC           PHOTODOCUMENTATION

High Performance Thin Layer Chromatography (HPTLC) is a visual chromatographic technique. What do we mean by the visual approach? The chromatograms, i.e., HPTLC plates, are observed after development. The human eye can see visible spots/bands. If the bands are UV active, we need to observe them under UV. Hence, the HPTLC plate can be visually evaluated, which sets it apart from other chromatographic techniques. The results are monitored and documented.

How are the results observed/ documented?

HPTLC/TLC results are visual and can be observed by the HPTLC Photodocumentation system. For the HPTLC photodoc, CAMAG has modules such as CAMAG TLC visualizer 2. It has a digital CCD camera. The TLC visualizer electronically captures the high-resolution images of chromatograms under white light, UV 254nm, and under fluorescence. It merges three images to give the best image possible, aided by software-controlled visionCATS for documentation. The software has image enhancement tools. The data generated is stored and can be used for further evaluation. “Comparison viewer” is a powerful tool available in software through which bands/tracks from the same /different plates can be compared side by side.

Another TLC photodoc system available with CAMAG is UV Cabinet 4. It has a UV lamp to observe the chromatogram under 254nm and 366nm. A UV filter is provided in viewing windows for eyes protection. This is not a software-controlled module. It can be manually operated, and a person can capture chromatogram images through a mobile phone camera.

CAMAG Smartdigi, one of the TLC photodocumentation modules, is specially designed for the Indian consumer at a low cost. It has a provision for mounting a digital camera to capture images under white light, UV 254nm, and UV 366nm. The visual presentation of the complete chromatogram showing the UV active samples and ideal standards side by side is one of the most convincing arguments for adopting the HPTLC Photodocumentation system. No other technique can directly produce the result as a color image and make it available for visual evaluation.

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Chromatographic Fingerprinting of Herbal Products

 HPTLC technique can be used for herbal fingerprinting. Each plant has different fingerprint pattern. When the plant extract is run in a suitable mobile phase the separation pattern observed is herbal fingerprint of the said plant. HPTLC Fingerprinting is the representation of the phytochemical composition of a plant extract or formulation, in the form of an image at 254nm, 366nm and white light. It is sequence of peaks or zones of a chromatogram specific for a sample

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Camag TLC in india

CAMAG TLC is one of the pioneers and leaders in HPTLC Software and instrumentation research. This has taken Thin Layer Chromatography from a very powerful but non-standardized technique to HPTLC today.

Visit us on:  Camag TLC in india

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HPTLC lab in Mumbai

Anchrom is the leading HPTLC instruments supplier in India, from CAMAG Switzerland with applications in Herbals, Pharma, Forensics, Foods, Chemicals, etc.

HPTLC is the modern version of TLC wherein the principles are same but the practice is fully automated and GLP/USP/EP compliant. 

HPTLC can analyse most nonvolatile organic compounds. The key features of HPTLC analysis are it is simple and visible chromatographic technique. 

Very little sample preparation is required and dozens of samples can be analysed simultaneously with multiple detection modes with very low cost.

 Applications inlcude food, herbals, pharmaceuticals, speciality chemicals, forensics, etc.

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Camag TLC

Anchrom is the leading HPTLC instruments supplier in India, from CAMAG Switzerland with applications in Herbals, Pharma, Forensics, Foods, Chemicals, etc.

CAMAG TLC is one of the pioneers and leaders in HPTLC Software and instrumentation research. This has taken Thin Layer Chromatography from a very powerful but non-standardized technique to HPTLC today.

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CAMAG HPTLC PRO

A sophisticated form of the TLC technique, HPTLC or high-performance thin layer chromatography is super-efficient. The content of HPTLC includes the validated methods of both quantitative and qualitative analysis and this fulfills all the requirements as per quality to use it in an environment that is completely regulated.

 The steps of both the HPTLC applications and the TLC process are almost identical. However, the main difference in TLC and HPTLC lies in the separation plate’s characteristics. The plates used in HPTLC are based on the optimized silica gel 60, which has a significantly small size of particles as compared to that used in the classical TLC plates. This in turn lets it enjoy a smooth surface and a high packaging entity. This helps the same diffusion to reduce and in turn results in compact spots and bands. The small size of the particles and the thin layer increases the detection significantly and increases the speed of analysis.

 CAMAG HPTLC

 HPTLC is an automated analytical technique, which has a high separation power and is reproducible as compared to the classical TLC. The thin layer and high performance chromatography helps to facilitate a very responsive scanning technology that helps to document and assess chromatography faster. The HPTLC finds use in separating the components, which is dependent on the polarity with which they are separated in the mobile phase. It is used in order to evaluate the purity and the efficiency of many of the pharma products as well as its dosage forms.

CAMAG allows sample analysis that is fully automated. It offers an evaluation system for routine quality control.

 Application

The sample extracts are applied in the band form on the plates using an applicator that is software controlled. The final result quality is impacted by the precision of the volume applied, the application zones compactness and the exact positioning of the sample.

Development

There are chambers for automatic development that makes the development step less dependent on any interactions and makes it more reproducible. You can preset the developing distance, the chamber configuration, final drying and activation. The developed steps progress is monitored.

 Derivatization

Through the process of derivatization, the substances that do not have fluorophores or chromophores can be made detectable. The reagents that are required are transferred using the automated spraying to transfer it to the chromatogram.

 Detection

The hptlc chromatography applications are the images that get captured on the plate under a white or an ultraviolet light. In order to get an electronic image acquisition a digital camera is used to capture the polychromatic light that is visible. The data that is obtained can be archived, edited and evaluated.

  MS-Interface

The TLS-MS coupling helps in verification of the analytes chemical structure using the process of mass spectrometry. The analytes can be eluted directly to an MS or elute can be collected for any further analysis in the offline mode.

 Software Integration

The HPTLC Software vision CATS helps to organize the workflow of the HPLTC analysis. It controls the CAMAG instrument and helps to manage the data evaluation.

 Conclusion

CAMAG is the world leader and pioneer in HP-TLC software and instrumentation. It is designed to take an advantage of all the unique aspects.

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What is The Difference Between TLC & HPTCL?

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The 4 ways HPTLC is used in Modern World

HPTLC is the most preferred method of analysis in the modern world. In this article, we will start from the basics of HPTLC principle and tell you about its uses.

HPTLC is one type of planar chromatography, and is more useful than TLC and HPLC. Since HPTLC is an independent, it is not only an instrument TLC, but complete concept of standardize methodology.

Features of HPTLC-

If you have idea of HPTLC principle, it is also important to have complete understanding about features. Some of the features of HPTLC are-

●      Low maintenance cost

●      Visual detection due to its open system

●      Several analysts working simultaneously on the system

There are different ways HPTLC used in the modern world, but first, let’s get to know what chromatography is.

What is chromatography?

The technique chromatography separates different types of chemical mixtures which we have to analyze individually from a homogeneous mixture (that has the same proportion of its compound throughout any given sample)

HPTLC is an advanced version of TLC (thin layer chromatography) which is a type of liquid chromatography. HPTLC is based on the principle of absorption. It is better than TLC in a way that it provides better separation of components.

The basic aim of HPTLC is separation but after separation, we can identify, quantify and isolate our component using the method micro preparation

Uses of HPTLC

1.     Pharmaceutical Industries

HPTLC has proved its role in pharmaceutical industries. Pharmaceutical industries are responsible for the production of drugs and medicines. These drugs and medicines are to be consumed by patients in order to cure their illness. HPTLC determines the purity and quantity of preservatives (any natural or artificial agent that acts to preserve) and compounds responsible for mixtures activity. HPTLC also has the ability to analyze vitamins in a given sample of a drug or medicine.

2.     Forensic Analysis

Forensic analysis uses science and technology in the investigation and establishment of evidences. Forensic scientists usually collect and analyze scientific evidences during the course of an investigation. The extensive role of HPTLC in separation of mixtures is helpful in forensic science. HPTLC is used in identification and comparison of different types of samples, be it drugs, explosives or even biological samples. HPTLC has been especially proved helpful in detecting cannabis (a mildly euphoriant or sedating drug)

3.     Food Analysis

Food is one of the fundamental part of any organism's life and the quality of the food one consuming, plays an important role in his growth. Food analysis is a scientific process which studies the chemical composition of food, how that food will affect the body, its calorific value, processing and several properties. The main aim of food analysis is to confirm, how safe the food is. HPTLC is helpful in detecting, if there is any kind of adulteration in the food. HPTLC can also check if there is any kind of illegal substitution of one substance for another

4.     Cosmetic Analysis

Cosmetics are widely used across the globe. They are meant to be applied on specific external parts of body (like nails, skin, hair etc) in order to protect, nourish, beautify and soften that part. Cosmetics contain numerous chemical components. These chemicals can even be toxic or harmful. Harmful effects may include damage of nails, removal of hairs, permanent dark circles. Hence, it becomes necessary to check the safety of these cosmetics. HPTLC technique can be employed for the detection and identification of such harmful chemicals and toxicants.

Parting Notes

It’s wonderful how to know how chromatography has come a long way in solving real world problems. There are several companies working towards making HPTLC principle the first and most preferred method of analysis in all markets they operate in. We hope they change the world through their initiative.

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HPTLC fingerprinting | Forensic Analysis, Application- Anchrom

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HPTLC Specialists | Forensic Analysis, Application- Anchrom