Backward Incompatible @backwardincompatible - Tumblr Blog

Missing horsey days… #bigmare #thelife #inthesaddle

For those of you wondering what I have been keeping myself busy with the past two years...

After a lot of debugging, simulating and benchmarking, the fastest and probably most accurate DNA barcode classifier (or demultiplexer, depending on your field)

has been released, packaged in BioConda and published in BMC Bioinformatics. https://biosails.github.io/pheniqs/

#dna barcode classifier #dna barcode decoding #bayesian #split-seq #split-pool #dna demultiplexing #rna-seq #bioconda #conda package

Bird, you are so pretty. #cateatinggrass #catonwindow #catonwindowsill #cateating #happycat #wheatgrass https://www.instagram.com/p/CKHDXGqJX2ZNeqljTvGDp50s3g7jtv_Tu3Tw5g0/?igshid=f1drtoitb0v6

#cateatinggrass #catonwindow #catonwindowsill #cateating #happycat #wheatgrass

The #stelthbomber of #insects. The stuff that happens when you move from #manhattan to the #suburbs https://www.instagram.com/p/CBrnezDJvvdQs4m9XQRo4JVPflQPcFnh-E1PzA0/?igshid=f8wiqoqscuc1

#stelthbomber #insects #manhattan #suburbs

Let me just get something from the back... #roadtrip #catinacar (at Niagara Falls, Ontario) https://www.instagram.com/p/B6eKhQqp9BYkodLlDbN0f_bAVPc5X7a2gUJSTg0/?igshid=pd26woq22zvw

#roadtrip #catinacar

“Let’s take the subway” she said. “It will be fun.” She said. #Sweating in the #subway. (at Delancey Street/Essex Street) https://www.instagram.com/p/B1XupOVJIQlnH9A156Mr2aR0Tz82YTE4YhP6FA0/?igshid=11224foqhw457

#sweating #subway

Afternoon in #sheepsmeadows (at Sheep's Meadow) https://www.instagram.com/p/B1Cn1AOJKsawkZOluz5ldPSuGfVnsj6uKRdKWo0/?igshid=j9doaeqc4wr6

#sheepsmeadows

#shimano #alfine sg-s7000-8 loose spring on the small clutch carrier for the first and fifth gears. A month of scratching my head and @ginjerbiscuit nagging that her bike is broken. Kudos to Andrew Kashutin for his video and identifying this obscure fault https://youtu.be/ynHJIGTvcwg

#shimano #alfine #spring

So... turns out the wippermann connex 10SB does not like being cleaned with mineral spirits

#wippermann #connex #bike chain #10SB #mineral spirits

Compiling Illumina bcl2fastq 2.20 on ubuntu with newer versions of boost

compiling the latest Illumina bcl2fastq2 version 2.20 and 2.19 on ubuntu 16.04.3 seems to yield the following error:

https://gist.github.com/moonwatcher/5698a70fe4be81d1277ee0296c4c8b0c

The error seems to stem from src/cxx/lib/io/Xml.cpp assuming that all non win32 systems use an old version of boost that did not templatize the boost::property_tree::xml_writer_make_settings function.

https://gist.github.com/moonwatcher/ab22ba80f7583d28282ebd8f1d9f7d23

Why Illumina makes such a preposterous assumption is besides the point but if you want to get bcl2fastq2 2.20.0 to build you can just do away with said assumption by applying this patch

https://gist.github.com/moonwatcher/ac1176971d6032fc9e0d4402e5a81333

For extra credit here is a zsh script for downloading, patching and building bcl2fastq2 2.20.0

https://gist.github.com/moonwatcher/2a97f1e1c795f99c390e608028587cc3

You still need to make sure your system has all the dependencies installed as documented in the code but you will end up with functioning binary bcl2fastq executable.

#bcl2fastq #bcl2fastq2 #illumina #ubuntu #POSIX #dna #sequencing #boost #linux

Watching #Godfather in #centralpark. Michel just shot virgil sollozzo and Sterling Hayden. Everyone clasped their hands, congratulating Al Pacino for a kill well done. I love #nyc. (at Central Park)

#centralpark #godfather #nyc