Molecular Structure of DNA and RNA, vocabulary.
Transformation: (1) when a plasmid vector or segment of chromosomal DNA is introduced into a bacterial cell; (2) when a normal cell is converted into a malignant cell.
32P & 35S: Radioactive isotopes of Phosphorus and Sulfur used to specifically label phage proteins and DNA.
Nucleotide: The repeating structural unit of nucleic acid, composed of a sugar, phosphate and base.
Ribose: A sugar found in RNA. (Ribonucleic acid)
Deoxyribose: A sugar found in DNA. (Deoxyribonucleic acid)
5' & 3' (Five & Three Prime) Carbons: Indicates the carbon number in the DNA's sugar group. 5' is attached to the phosphate where as 3' is attached to a hydroxyl group.
Double Helix: The arrangement in which two strands of DNA (sometimes RNA) interact with each other to form a double-stranded helical structure.
Purine: A type of nitrogenous base that has a double ring structure. Such as, adenine and guanine.
Pyrimidine: A type of nitrogenous base that has a single-ring structure. Such as, cytosine, thymine and uracil (found in RNA).
Phosphodiester Bond: In a DNA or RNA strand, a linkage in which a phosphate group connects two sugar molecules together.
Anti-Parallel: An arrangement in a double helix in which one strand is running in the 5' to 3' direction and the other stand is 3' to 5'.
Right-Handed Helix: The clockwise motion a DNA double helix strand spirals.
Left-Handed Helix: Counter-clock wise spiraling of DNA.
Major Groove: A wide indentation in the DNA double helix in which the bases have access to water.
Minor Groove: A narrow indentation in the DNA double helix in which the bases have access to water.
A-DNA: A right handed DNA double helix with 11 base pairs per turn. Does not occur in living cells.
B-DNA: The predominant form of DNA in living cells. It is a right-handed DNA helix with 10 base pairs per turn.
Z-DNA: A left-handed DNA double helix that is found occasionally in the DNA of living cells.
Complementary Bases: In DNA, cytosine always pairs with guanine and thymine always pairs adenine. This is known as the AT/GC rule. In RNA, thymine is replaced with uracil and uracil now pairs up with adenine.