Under The Microscope

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redit Vincent Pasque, University of Cambridge, Wellcome Images  Xenopus laevis oocytes  Stage V-VI Xenopus laevis oocytes surrounded by thousands of follicle cells, as visualized by Hoechst staining. Fluorescence microscopy 2009  Collection: Wellcome Images

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Interesting & important news in regards to the famous HeLa cell line. 

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An interesting video on cancer cells and their properties.  Enjoy!

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Human mesenchymal stem cells labelled with a dye called phalloidin which stains a cytoskeletal protein, actin. The nuclei are counter-stained. The cell cytoskeleton is the cells scaffolding, structuring the cell and in some cells, providing movement or shuttling of different proteins. (Image taken by Tony Rossetti)

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Category: High-content analysis. Image description: Ovarian cancer cell culture. Therapeutic focus: Cancer.

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Category: High-content analysis. Image description: Trisomy 21 derived neural cells stained for neurofilament heavy (red), GAD65 (green) and DNA (blue). Therapeutic focus: Down's Syndrome.

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Add these!

You should add some pictures from here to your blog!

https://promo.gelifesciences.com/na/K12301/gallery.asp#.UUU2DRwWnY4

This is great! Everyone check this out, I'll add some of these!

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Confocal image of septuple in situ hybridization exhibiting the spatial expression of Hox gene transcripts in a developing Drosophila embryo, with the chromosomal organization of the Hox gene cluster shown below: lab labial, pb proboscipedia (not shown in the in situ hybridization), Dfd deformed, Scr sex combs reduced, Antp antennapedia, Ubx ultrabithorax, Abd-A abdominal-A, Abd-B abdominal-B.

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A time-lapse, confocal micrograph captures the swirled cell division of a (HeLa) cancer cell with its cell membrane shown in cyan and DNA in red. Kuan-Chung Su, LRI, Wellcome Images

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Retinal Pigment Epithelium

Confocal image of rat RPE layer two weeks after laser. A well-defined neovascular membrane labeled with Isolectin Ib4 (red) is detected below proliferating RPE cells (Phalloidin in green). New vessels are growing toward the subretinal space.

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Clostridium botulinum

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This movie shows the "yolk flash". The apparently whole-embryo calcium pulse appears to initiate over a widespread area of blastoderm, possibly earliest at the animal pole, and then spreads first to the yolk cell membrane and then along the ventral midline heading rostrally. 30-sec integration window moving in 5-sec steps, elapsed real time of 340 sec played at 50x real time.. Fertilized zebrafish eggs were collected within 5 min of spawning, enzymatically dechorionated, and injected with approximately 0.9 nl of a 1% solution of recombinant f-aequorin in 100 mM KCl, 5 mM Mops, and 50 μM EDTA. During imaging the embryos were maintained at 28°C in 30% Danieau's medium containing penicillin (0.5 mg/ml), streptomycin (5,000 units/ml; Sigma), and 0.5% methylcellulose. Imaging was performed on a Photon Imaging Microscope (Science Wares, Falmouth, MA) that used a photon-counting spatial detector with a resistive anode output (Photek, St. Albens-on-Sea, U.K.). Digitized detector output in the form of a stream of time-labeled eight-bit x--y coordinates (256 × 256 pixels) was used to construct time-lapse imaging sequences. The imaging system software allowed the original photon data stream to be analyzed according to any chosen integration time, with the resulting image frames maintaining accurate photon quantitation up to 256 photons per pixel. These images are part of an image series within the Zebrafish--The Living Laboratory CD made available by Mark Cooper and described in Methods in Cell Biology Volume 77, 2004, Pages 439-457. Corresponds to supplemental video in PNAS January 5, 1999 vol. 96: 157-161 and quantification of color scale shown in Fig 1.

Source 1. Source 2.

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Fluorescence micrograph of a neuron regenerating in culture. From Aplysia.

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Some cheek cells of mine under phase contrast and fluorescent microscopy (400x). Poor quality but fun nonetheless. Shame there was too much bleed through with the MitoTracker.

Clockwise from top left: phase contrast, MitoTracker dye, WGA, DAPI

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Tip of a Butterfly's Tongue

Thanks fencingbanana!

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Bubble Wrap Injected with Ink

Thanks fencingbanana!