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Evaporative Light Scattering Detectors have been used in analytical laboratories for almost 40 years. Many applications using ELSD for detection can be found in journals specializing in chromatography. The SofTA ELSD's can perform these applications with similar or improved results. We will assist you in converting the ELSD conditions cited in an article or application note for our exclusive Thermo-split™ technology. Online search engines are wonderful resources for finding chromatographic applications. Follow the link below for more information.

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ELSD Profile of Japanese Sake

These overlaid chromatograms compare the profile of Japanese Sake analyzed with ELSD, UV at 254 nm and 210 nm.

This analysis was performed in order to detect amino acids in Sake using the SofTA ELSD.  Because Sake contains various sugar-related compounds, it is difficult to find complete separation condition. However, this chromatogram demonstrates that the SofTA ELSD provides a chromatographic profile of a beverage that contains many compounds that have no UV absorbency.

Note: Data has been normalized to bring UV profile at 210nm on scale

Sample: Japanese Sake

Column: Hypercarb 4.6 x 100 mm 10°C

Mobile phase; 20mM NFPA, 20mM NFPA containing 70 % CH3CN Gradient

Flow rate: 0.8 ml/min

SofTA ELSD:  Spray Chamber 15°C Drift Tube 50°C, FLT=6, Gain=Normal, 5VFS

UV: 254 nm & 210 nm

Chromatogram provided by Miyako Kawakatsu and Hiroyuki Kaiho, M&S Instruments

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Ceramide Mixture

HPLC condition

Sample: Ceramide mixture (about 500 ng each)

Column: Betasil DIOL100（4.6mm ID Ｘ150mm L）

Column temp.: 35°C

Mobile phase: A: Chloroform  B: 5％H2O/MeOH

Gradient: 0min→1min→10min、7 ％B→7 ％B→50％B

Flow rate: 1.2ml/min

SofTA ELSD Conditions: Spray Chamber 45°C Drift Tube 55°C Filter 3, Gain Normal

Chromatogram provided by Miyako Kawakatsu and Hiroyuki Kaiho, M&S Instruments

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Underivatized Amino Acids

Column: HyperCarb,

Mobile Phase: A: Nonafluoropentanoic acid B: ACN/ Nonafluoropentanoic acid

Sample: 10nM Each Amino Acid

Peaks: 1:Gly, 2: Ser 3: Ala, 4: Thr, 5: Asn, 6: Pro, 7: Asp 8: Gaba, 9: Gln, 10: E, 11: V, 12: Lys, 13: Leu, 14: Cys, 15: Met, 16: Ile, 17: His, 18: Arg, 19: Phe, 20: Tyr, 21: Impurity, 22: Trp

SofTA ELSD

Spray Chamber: 30°C

Drift Tube: 55°C

Filter: 6

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Gradient Ion Chromatography

Column: SCX

Mobile Phase: A: Water B: Ammonium Formate

Peaks: 1: Cl, 2: Na, 3: K, 4: Mg, 5: Ca

SofTA ELSD

Spray Chamber: 10°C

Drift Tube: 55°C

Filter: 6

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Triglycerides

Column: C18

Mobile Phase: A: Acetonitrile B: Dicloromethane

Peaks: 1: LnLnLn 2: LLL, 3: OOO

SofTA ELSD

Spray Chamber: 60°C

Drift Tube: 60°C

Filter: 3

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Macadamian Nut Oil

Column: C18

Mobile Phase: A: Acetonitrile B: Dicloromethane

Sample: 3µl of 0.5%

SofTA ELSD

Spray Chamber: 60°C

Drift Tube: 60°C

Filter: 3

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SofTA ELSD Comparison to Other ELSD and MS

Comparing ELSDs from different manufacturers is a difficult and involved process.  Each design has unique benefits and disadvantages, so no single test can completely do the job.  However, time and time again, our customers have told us that they get the best results with SofTA products.

 At SofTA, we have designed a very flexible instrument configuration that yields real advantages in many common HPLC applications.  One unique SofTA advantage is the ability to optimize for gradient operation.  This is possible because we can smoothly vary the vapor phase split ratio with our Thermo-Split technology.

The following data was customer generated during a comparative demonstration.  The customer had recently changed from brand “S” to brand “P” over the issue of sensitivity.  We were invited to run a battery of 10 tests versus their new ELSDs.  For all analytes and conditions tested, SofTA out-performed the competition.

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ELSD Comparison with Narrowbore Application

Comparing ELSDs from different manufacturers is a difficult and involved process.  Each design has unique benefits and disadvantages, so no single test can completely do the job.  However, time and time again, our customers have told us that they get the best results with SofTA products.

 At SofTA, we have designed a very flexible instrument configuration that yields real advantages in many common HPLC applications.  One unique SofTA advantage is the ability to optimize for gradient operation.  This is possible because we can smoothly vary the vapor phase split ratio with our Thermo-Split technology.

This comparison was made between the SofTA Model 300 and one of the other available ELSD's.  The SofTA design was able to detect this compound with better signal to noise ratio less band broadening.

SofTA Model 300

ELSD Brand "S"

Sample: HET, 100ng

Column: Hichrom RPB, 5µm,  2.1  x 50 mm

Mobile phase: 15% MeCN/Water

Flow rate: 0.2 ml/min

SofTA ELSD:  Spray Chamber 25°C Drift Tube 50°C, FLT=5

Brand "S": 3.5 bar, 50°C, Gain 12

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Starch Hydrolysate and Glucose Oligomer

Column: Asahipak NH2P 4.6mm x 150mm

Column Temperature: 40°C

Mobile Phase: A: CH3CN  B: Water

Gradient: 0 min 25% B, 18 min 40% B, 35 Min 80% B

Flowrate: 1ml/min

ELSD:  SofTA Model 200

Spray Chamber Temperature: 25°C

Drift Tube Temperature: 45°C

Gain: Normal

Full Scale:5V

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Monitoring of Synthesized Compound with ELSD

The following data  shows usefulness of the SofTA ELSD compared with UV detector. As G-OMe does not have UV absorbance, it is difficult to monitor this synthesis reaction with UV detector.

G-OMe(Glycine methylester) + Boc-F (Butoxy phenylalanine) = Boc-F-G-OMe

Column; BETASILC18 4.6 x 150mm L

Col. Temp.; Ambient

Mobile Phase; A_H2O, B_MeOH

Gradient; 0 min 40 % B, 7 min 100 % B, 15 min 100 % B

Flow Rate; 1 ml/min

SofTA conditions;　Blue line

SC temp.; 45 C

DT temp,; 60 C

FLT; 0

UV; 254 nm (0.001 au 10mV) Red line

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