S1 Ep 3
Vash is firing his 22 toward the dot cord representation of Nai's CG chain of knives, shouting, "Chance number two... Put the plant down!"
Nai slowly turns to his left side to look down at Vash, smirking. "You would shoot me?"
He doesn't give any sign of uneasiness as he sees Vash rummaging through his coat. He wonders for a split second if the missing bullets gag was being repeated but rolls with it and continues his line. "Your own big brot-"
He freezes when he sees what Vash is up to, and yells internally at his muscles to move, but Vash is already pointing a nerf gun at him and shoots him square in the forehead before he can do anything but blink.
The crew devolves into fits of laughter as Nai delivers a wry smile, the kind that promised payback was coming.