Apr 20, 2012
"Our laboratory has developed a fabrication system for functional 3D tissues by stacking cell sheets of confluent cultured cells detached from a temperature-responsive culture dish. Here we describe the protocols for the fabrication of 3D tissues by cell sheet engineering."
Source: Yuji Haraguchi, et al. Fabrication of functional three-dimensional tissues by stacking cell sheets in vitro. Nature Protocols 7 , 850–858 (2012).