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In my co-immunoprecipitation research, how can I set up a control?
In my co-immunoprecipitation research, how can I set up a control? Controls are essential to ensuring the specificity and validity of your co-immunoprecipitation experiment. Several forms of regulation are listed below. 1. IgG controls are negative controls; you should add an IgG control for non-specific binding. An IgG secondary antibody should not recognize any of the components in your…
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BioAdvisers said on Biotech Advisers
Essential guidance 2 | Fishing with Purekine "fishing gear", Protein Association "wishers hooked"
In the last issue, the editor brought you “Dry Goods | Using IP “Hook” Fishing, Protein Club “Wishers Take the Bite””, and the response was very enthusiastic. In the voice of everyone, the editor is rushing through the previous experimental notes, bringing you the second bullet of protein interaction knowledge-Pull-Down. If the protein interaction is compared to an interesting “fishing” activity, then the Purekine filler we use is a tough and slender “fishing rod” and “fishing line”. The known GST-labeled recombinant protein is sharp The strong “fish hook” and “fish bait” are the perfect combination, and our target protein is the “fish” that moves freely in the water.
The concept of Pull-Down is similar to Co-IP, and its purpose is to study proteins or ligands that bind to known bait proteins. Pull-Down aims to prove the interaction between two proteins or explore unknown proteins or molecules that can bind to the target protein. Pull-Down is different from IP or Co-IP in that it is not based on antibody-antigen interaction, not an immune response. The bait protein (or ligand) is immobilized on a solid support through a non-antibody affinity system. This immobilization can be covalently coupled to the activated microbeads, or through the receptor molecule on the support The bound affinity tag binds and thus immobilizes. For example, immobilized metal chelating affinity chromatography (IMAC) resin can be used to pull-down histidine tag bait protein. The optimization of Pull-Down needs to consider the special nature of the affinity system used. Principle of Pull-Down According to the following diagram, you can understand the principle of Pull-Down:
Comparison of Pull-Down and protein purification experiments:
Use recombinant gene expression protein, and then perform downstream protein extraction and purification experiments；
Analysis of the similarities and differences between the two experimental procedures；
Experiment type Pull-Down protein purification Solid carrier Affinity chromatography resin Affinity chromatography resin filler Carrier form In bulk Column Incubation method Shaker incubation Incubate in the purification column Type of buffer Binding Buffer
Wash Buffer
Elution Buffer
Binding Buffer
Wash Buffer
Elution Buffer
Target protein form Prey protein Recombinant protein
Types of products to be tested:
Product Category Item No. His tag protein purification tool BMR20000 GST tag protein purification tool BMR20100 MBP tag protein purification tool BMR20206 Flag tag protein purification tool BMR21504 Biotin tag protein purification tool BMR20306 Protein A Antibody purification tools BMR21604 Protein GAntibody purification tools BMR20600 Protein A/G Antibody purification tools BMR20704 Protein L Antibody purification tools BMR20800
Conclusion
The Purkine series of protein purification tools provided by Abbkine are multifunctional and suitable for a variety of protein experiments. When your target protein is a recombinant protein, the purification medium can be used for the extraction of recombinant protein, which is a protein purification experiment; when your target protein is a prey protein that interacts with the recombinant protein, this series of products can be used for prey Extraction and purification.
This issue mainly talks about the knowledge of Pull-Down, time is limited, I have to say goodbye to everyone. At the same time in the next issue, we will have exciting content to meet you all. Stay tuned~~~~~
You are welcome to pay more attention to Abbkine’s official account, and there will be more scientific research surprises waiting for you. At the same time, in order to provide readers with a platform for learning biological knowledge, Abbkine’s technical experts have worked tirelessly to sort out the dry goods of a variety of high-end biological experiments, which will be published in the WeChat public account in the later period. Please continue to pay attention to Abbkine for readers who love science. No public.
Abbkine focuses on the fields of proteinology and cytology, and is committed to the innovation and research and development of various antibodies, proteins, analytical reagents and kits, in order to become a key promoter in the development of life science research, drug development and other fields. We provide you with the favorite products of protein and immune research users, from basic immunological products, such as protein extraction and quantification, to internal reference label antibodies, primary and secondary antibodies for immunological experiments; the favorite products of cell research users, from Dyes and kits for detecting cell status, organelle extraction kits, cell substructure staining and tracking and cell metabolism detection products, to cytokine and protein detection kits for cell culture, just to help your research career !
Bioadvisers shared on Biotech Advisers
Essential guidance 2 | Fishing with Purekine "fishing gear", Protein Association "wishers hooked"
In the last issue, the editor brought you “Dry Goods | Using IP “Hook” Fishing, Protein Club “Wishers Take the Bite””, and the response was very enthusiastic. In the voice of everyone, the editor is rushing through the previous experimental notes, bringing you the second bullet of protein interaction knowledge-Pull-Down. If the protein interaction is compared to an interesting “fishing” activity, then the Purekine filler we use is a tough and slender “fishing rod” and “fishing line”. The known GST-labeled recombinant protein is sharp The strong “fish hook” and “fish bait” are the perfect combination, and our target protein is the “fish” that moves freely in the water.
The concept of Pull-Down is similar to Co-IP, and its purpose is to study proteins or ligands that bind to known bait proteins. Pull-Down aims to prove the interaction between two proteins or explore unknown proteins or molecules that can bind to the target protein. Pull-Down is different from IP or Co-IP in that it is not based on antibody-antigen interaction, not an immune response. The bait protein (or ligand) is immobilized on a solid support through a non-antibody affinity system. This immobilization can be covalently coupled to the activated microbeads, or through the receptor molecule on the support The bound affinity tag binds and thus immobilizes. For example, immobilized metal chelating affinity chromatography (IMAC) resin can be used to pull-down histidine tag bait protein. The optimization of Pull-Down needs to consider the special nature of the affinity system used. Principle of Pull-Down According to the following diagram, you can understand the principle of Pull-Down:
Comparison of Pull-Down and protein purification experiments:
Use recombinant gene expression protein, and then perform downstream protein extraction and purification experiments；
Analysis of the similarities and differences between the two experimental procedures；
Experiment type Pull-Down protein purification Solid carrier Affinity chromatography resin Affinity chromatography resin filler Carrier form In bulk Column Incubation method Shaker incubation Incubate in the purification column Type of buffer Binding Buffer
Wash Buffer
Elution Buffer
Binding Buffer
Wash Buffer
Elution Buffer
Target protein form Prey protein Recombinant protein
Types of products to be tested:
Product Category Item No. His tag protein purification tool BMR20000 GST tag protein purification tool BMR20100 MBP tag protein purification tool BMR20206 Flag tag protein purification tool BMR21504 Biotin tag protein purification tool BMR20306 Protein A Antibody purification tools BMR21604 Protein GAntibody purification tools BMR20600 Protein A/G Antibody purification tools BMR20704 Protein L Antibody purification tools BMR20800
Conclusion
The Purkine series of protein purification tools provided by Abbkine are multifunctional and suitable for a variety of protein experiments. When your target protein is a recombinant protein, the purification medium can be used for the extraction of recombinant protein, which is a protein purification experiment; when your target protein is a prey protein that interacts with the recombinant protein, this series of products can be used for prey Extraction and purification.
This issue mainly talks about the knowledge of Pull-Down, time is limited, I have to say goodbye to everyone. At the same time in the next issue, we will have exciting content to meet you all. Stay tuned~~~~~
You are welcome to pay more attention to Abbkine’s official account, and there will be more scientific research surprises waiting for you. At the same time, in order to provide readers with a platform for learning biological knowledge, Abbkine’s technical experts have worked tirelessly to sort out the dry goods of a variety of high-end biological experiments, which will be published in the WeChat public account in the later period. Please continue to pay attention to Abbkine for readers who love science. No public.
Abbkine focuses on the fields of proteinology and cytology, and is committed to the innovation and research and development of various antibodies, proteins, analytical reagents and kits, in order to become a key promoter in the development of life science research, drug development and other fields. We provide you with the favorite products of protein and immune research users, from basic immunological products, such as protein extraction and quantification, to internal reference label antibodies, primary and secondary antibodies for immunological experiments; the favorite products of cell research users, from Dyes and kits for detecting cell status, organelle extraction kits, cell substructure staining and tracking and cell metabolism detection products, to cytokine and protein detection kits for cell culture, just to help your research career !
BioAdvisers said on Biotech Advisers
Using immunoprecipitation "fish hooks" for fishing, the protein will always "hooked with willing"
The thoughtful Thursday is here again, and the editor who has long been unable to bear it is finally released to meet our favorite “Yin Fans”.
This time, the editor brings you a wave of colorful biological science mysteries-protein interaction. If protein interaction is compared to an interesting “fishing” activity, then the tag antibody coupling carrier we use is a sharp and strong “fish hook”, and the tag recombinant protein (bait protein) is sweet and delicious. “Bait”, and our target protein is the “fish” that moves freely in the water.
If you want to do your job well, you must first sharpen your tools. Maybe you are still worrying about the purification and enrichment of the target protein without a good antibody coupled to Beads. Maybe the protein content you collected at this time is low, maybe you are enriching it at this time. The protein impurity content is high, maybe the protein vitality that you have harvested through the hard work at this time has been reduced, maybe the Beads operation steps you are using now are long and take a lot of time, maybe, maybe…
Next, the editor will give you a feast of protein experiments: reveal the whole process of protein interaction, what kind of magic operation is IP and Co-IP?
Immunoprecipitation (IP)
“Immunoprecipitation” generally refers to a small-scale protein affinity purification experiment using a binding protein immobilized on a solid support. More precisely, IP is an experiment in which a specific antibody immobilized on a microbead support (usually agarose resin) is used to purify a single antigen from a complex mixture. The assembly of the immobilized protein complex can be performed step by step or completed in one step (Figure 1). Common loading sequence: incubate the antibody and sample (such as cell lysate) together, and then add affinity beads to capture the antibody-antigen complex. It is also possible to incubate the antibody and microbeads (directly or indirectly bind to the antibody through the antibody binding protein, such as protein A, G, or AG), and then add the sample containing the antigen. After the antigen, antibody and solid support are bound, the beads are washed thoroughly, and then an appropriate elution buffer is used to elute the antigen from the support.
Co-immunoprecipitation(Co-IP)
Co-immunoprecipitation analysis (Co-IP) is very similar to IP. The basic technique is to use immobilized antibodies specific to the target antigen; however, the goal of IP is to purify a single antigen, while Co-IP is designed to separate the antigen and the antigen. Bound protein or ligand. In the Co-IP experiment, the known antigen is called the bait protein, and the protein bound to it is called the target protein. The target protein may be some complex chaperone proteins, signal molecules, structural proteins, cofactors, etc. The intensity of the interaction between proteins may range between highly transient and very stable. The basic Co-IP experiment scheme is the same as that of IP. In fact, any IP system can be used for Co-IP. However, there are many other factors that need to be considered, for example, the optimization of binding and washing conditions. When optimizing, the strength of the bait protein-target protein interaction and the affinity of the antibody-bait protein need to be considered.
Disadvantages of Co-IP experiment:
Low-affinity and instant protein-protein interactions may not be detected; the binding of the two proteins may not be a direct binding, but a third party may act as a bridge in the middle; the target protein must be predicted before the experiment to choose the final Antibodies tested, so if the prediction is incorrect, the experiment will have no results.
Advantages of Co-IP experiment:
The interacting proteins are all post-translationally modified and are in a natural state; protein interactions are carried out in a natural mixed protein state, which can avoid human influence; the interacting protein complex in the natural state can be separated.
Technical upgrade of IP and Co-IP
Basic steps of traditional IP and Co-IP:
Add the appropriate antibody.
The antibody binds to the target protein.
Add Protein A/G to make the antibody-protein complex insoluble.
Centrifuge the solution to separate the antibody-protein complex.
Remove the supernatant and wash.
Limitations of traditional ProteinA/G in IP applications:
ü1) Time-consuming
ü2) When the antibody species, subtype and ProteinA/G do not match, the ability of ProteinA/G to bind to the antibody is weak
ü3) Binding with other immunoglobulins in the sample, resulting in non-specific binding, resulting in high background
Basic steps of IP and Co-IP after upgrade:
Add the appropriate antibody conjugate.
The antibody binds to the target protein.
Centrifuge the solution to separate the antibody-protein complex.
Remove the supernatant and wash.
After using our upgraded agarose/magnetic bead coupling tag antibody, we can be surprised to find that the experimental process eliminates a time-consuming and complicated experimental operation of antibody and carrier binding, and the perfect combination of antibody and Beads , Improve the accuracy of the target protein being “pulled”, the purity and yield of the target protein obtained will be significantly improved, and the operation is simple, saving time and effort!
Having said that, you must be impatient for our Abbkine’s “protein weapon”. The editor is now very proud to recommend our agarose/magnetic bead conjugated antibody to everyone:
Cat.NO Product name Application A02010AGB Anti-DDDDK Tag Mouse Monoclonal Antibody, Agarose) IP/Co-IP A02010MGB Anti-DDDDK Tag Mouse Monoclonal Antibody, Magnetic Beads) IP/Co-IP A02040AGB Anti-HA Tag Mouse Monoclonal Antibody, Agarose IP/Co-IP A02040MGB Anti-HA Tag Mouse Monoclonal Antibody, Magnetic Beads IP/Co-IP A02050AGB Anti-His Tag Mouse Monoclonal Antibody, Agarose IP/Co-IP A02050MGB Anti-His Tag Mouse Monoclonal Antibody, Magnetic Beads IP/Co-IP A02060AGB Anti-Myc Tag Mouse Monoclonal Antibody, Agarose IP/Co-IP A02060MGB Anti-Myc Tag Mouse Monoclonal Antibody, Magnetic Beads IP/Co-IP A02170AGB Anti-V5 Tag Mouse Monoclonal Antibody, Agarose IP/Co-IP A02170MGB Anti-V5 Tag Mouse Monoclonal Antibody, Magnetic Beads IP/Co-IP
This issue is mainly about the knowledge of immunoprecipitation, time is limited, I have to say goodbye to everyone again. At the same time in the next issue, there will be Pull down operation tips with protein interaction to meet you. Stay continued~~~~~
You are welcome to pay more attention to the Abbkine, and there will be great scientific research surprises waiting for everyone. At the same time, in order to provide readers with a platform for learning biological knowledge, Abbkine’s technical experts have, after unremitting efforts, sorted out the dry goods of a variety of high-end biological experiments, and later published them in the WeChat public account. Please continue to pay attention to Abbkine for readers who love science. the public.
Abbkine focuses on the fields of proteinology and cytology, and is committed to the innovation and research and development of various antibodies, proteins, analytical reagents and kits, in order to become a key promoter in the development of life science research, drug development and other fields. We provide you with the favorite products of protein and immunological research users, from basic immunology products, such as protein extraction and quantification, to internal reference label antibodies, primary antibodies and secondary antibodies for immunological experiments; cell research users’ favorite products, from Dyes and kits for detecting cell status, organelle extraction kits, cell substructure staining and tracking and cell metabolism detection products, to cytokine and protein detection kits for cell culture, are only for your research career !
Bioadvisers shared on Biotech Advisers
Using immunoprecipitation "fish hooks" for fishing, the protein will always "hooked with willing"
The thoughtful Thursday is here again, and the editor who has long been unable to bear it is finally released to meet our favorite “Yin Fans”.
This time, the editor brings you a wave of colorful biological science mysteries-protein interaction. If protein interaction is compared to an interesting “fishing” activity, then the tag antibody coupling carrier we use is a sharp and strong “fish hook”, and the tag recombinant protein (bait protein) is sweet and delicious. “Bait”, and our target protein is the “fish” that moves freely in the water.
If you want to do your job well, you must first sharpen your tools. Maybe you are still worrying about the purification and enrichment of the target protein without a good antibody coupled to Beads. Maybe the protein content you collected at this time is low, maybe you are enriching it at this time. The protein impurity content is high, maybe the protein vitality that you have harvested through the hard work at this time has been reduced, maybe the Beads operation steps you are using now are long and take a lot of time, maybe, maybe…
Next, the editor will give you a feast of protein experiments: reveal the whole process of protein interaction, what kind of magic operation is IP and Co-IP?
Immunoprecipitation (IP)
“Immunoprecipitation” generally refers to a small-scale protein affinity purification experiment using a binding protein immobilized on a solid support. More precisely, IP is an experiment in which a specific antibody immobilized on a microbead support (usually agarose resin) is used to purify a single antigen from a complex mixture. The assembly of the immobilized protein complex can be performed step by step or completed in one step (Figure 1). Common loading sequence: incubate the antibody and sample (such as cell lysate) together, and then add affinity beads to capture the antibody-antigen complex. It is also possible to incubate the antibody and microbeads (directly or indirectly bind to the antibody through the antibody binding protein, such as protein A, G, or AG), and then add the sample containing the antigen. After the antigen, antibody and solid support are bound, the beads are washed thoroughly, and then an appropriate elution buffer is used to elute the antigen from the support.
Co-immunoprecipitation(Co-IP)
Co-immunoprecipitation analysis (Co-IP) is very similar to IP. The basic technique is to use immobilized antibodies specific to the target antigen; however, the goal of IP is to purify a single antigen, while Co-IP is designed to separate the antigen and the antigen. Bound protein or ligand. In the Co-IP experiment, the known antigen is called the bait protein, and the protein bound to it is called the target protein. The target protein may be some complex chaperone proteins, signal molecules, structural proteins, cofactors, etc. The intensity of the interaction between proteins may range between highly transient and very stable. The basic Co-IP experiment scheme is the same as that of IP. In fact, any IP system can be used for Co-IP. However, there are many other factors that need to be considered, for example, the optimization of binding and washing conditions. When optimizing, the strength of the bait protein-target protein interaction and the affinity of the antibody-bait protein need to be considered.
Disadvantages of Co-IP experiment:
Low-affinity and instant protein-protein interactions may not be detected; the binding of the two proteins may not be a direct binding, but a third party may act as a bridge in the middle; the target protein must be predicted before the experiment to choose the final Antibodies tested, so if the prediction is incorrect, the experiment will have no results.
Advantages of Co-IP experiment:
The interacting proteins are all post-translationally modified and are in a natural state; protein interactions are carried out in a natural mixed protein state, which can avoid human influence; the interacting protein complex in the natural state can be separated.
Technical upgrade of IP and Co-IP
Basic steps of traditional IP and Co-IP:
Add the appropriate antibody.
The antibody binds to the target protein.
Add Protein A/G to make the antibody-protein complex insoluble.
Centrifuge the solution to separate the antibody-protein complex.
Remove the supernatant and wash.
Limitations of traditional ProteinA/G in IP applications:
ü1) Time-consuming
ü2) When the antibody species, subtype and ProteinA/G do not match, the ability of ProteinA/G to bind to the antibody is weak
ü3) Binding with other immunoglobulins in the sample, resulting in non-specific binding, resulting in high background
Basic steps of IP and Co-IP after upgrade:
Add the appropriate antibody conjugate.
The antibody binds to the target protein.
Centrifuge the solution to separate the antibody-protein complex.
Remove the supernatant and wash.
After using our upgraded agarose/magnetic bead coupling tag antibody, we can be surprised to find that the experimental process eliminates a time-consuming and complicated experimental operation of antibody and carrier binding, and the perfect combination of antibody and Beads , Improve the accuracy of the target protein being “pulled”, the purity and yield of the target protein obtained will be significantly improved, and the operation is simple, saving time and effort!
Having said that, you must be impatient for our Abbkine’s “protein weapon”. The editor is now very proud to recommend our agarose/magnetic bead conjugated antibody to everyone:
Cat.NO Product name Application A02010AGB Anti-DDDDK Tag Mouse Monoclonal Antibody, Agarose) IP/Co-IP A02010MGB Anti-DDDDK Tag Mouse Monoclonal Antibody, Magnetic Beads) IP/Co-IP A02040AGB Anti-HA Tag Mouse Monoclonal Antibody, Agarose IP/Co-IP A02040MGB Anti-HA Tag Mouse Monoclonal Antibody, Magnetic Beads IP/Co-IP A02050AGB Anti-His Tag Mouse Monoclonal Antibody, Agarose IP/Co-IP A02050MGB Anti-His Tag Mouse Monoclonal Antibody, Magnetic Beads IP/Co-IP A02060AGB Anti-Myc Tag Mouse Monoclonal Antibody, Agarose IP/Co-IP A02060MGB Anti-Myc Tag Mouse Monoclonal Antibody, Magnetic Beads IP/Co-IP A02170AGB Anti-V5 Tag Mouse Monoclonal Antibody, Agarose IP/Co-IP A02170MGB Anti-V5 Tag Mouse Monoclonal Antibody, Magnetic Beads IP/Co-IP
This issue is mainly about the knowledge of immunoprecipitation, time is limited, I have to say goodbye to everyone again. At the same time in the next issue, there will be Pull down operation tips with protein interaction to meet you. Stay continued~~~~~
You are welcome to pay more attention to the Abbkine, and there will be great scientific research surprises waiting for everyone. At the same time, in order to provide readers with a platform for learning biological knowledge, Abbkine’s technical experts have, after unremitting efforts, sorted out the dry goods of a variety of high-end biological experiments, and later published them in the WeChat public account. Please continue to pay attention to Abbkine for readers who love science. the public.
Abbkine focuses on the fields of proteinology and cytology, and is committed to the innovation and research and development of various antibodies, proteins, analytical reagents and kits, in order to become a key promoter in the development of life science research, drug development and other fields. We provide you with the favorite products of protein and immunological research users, from basic immunology products, such as protein extraction and quantification, to internal reference label antibodies, primary antibodies and secondary antibodies for immunological experiments; cell research users’ favorite products, from Dyes and kits for detecting cell status, organelle extraction kits, cell substructure staining and tracking and cell metabolism detection products, to cytokine and protein detection kits for cell culture, are only for your research career !
New Post has been published on Abbkine - Antibodies, proteins, biochemicals, assay kits for life science research
Abbkine announces the release of its new antibody - IPKine™ HRP Goat Anti-Mouse IgG HCS
Wuhan, China. 430074. 26th April 2017. Abbkine Scientific is no doubt one of the world’s leading scientific research companies and the institution recently announced the addition of another antibody into the IPKine family. The IPKine™ HRP Goat Anti-Mouse IgG HCS is an HRP Secondary Antibody designed to help researchers and scientists get the best out of their works without necessarily breaking the bank.
The Anti-Mouse Heavy Chain comes in a liquid solution with such features as heavy chain/Fc fragment specific optimization, helping to eliminate light chain interference. Its absorption of serum proteins also helps to minimize cross-reactivity with immunoglobulins from other species, which are some of its unique features.
The antibody reacts with Fc portion of mouse IgG heavy chain, while it doesn’t react with the Fab portion of mouse IgG. It's cross-reaction with human, bovine and horse serum proteins has been specialized minimized, while it may cross-react with immunoglobulins from other species. It has no reactivity on non-immunoglobulin serum proteins.
With its host being the goat, the antibody’s applications include WB, IP and others, hence a co-IP. For optimal working conditions, the dilutions should be determined by the investigator through experiment. However, the suggested solutions are WB 1:10,000-1:100,000, IHC 1:500-1:5,000 and ELISA 1:5,000-1:100,000.
The IPKine™ HRP Goat Anti-Mouse IgG HCS can be stored for as long as one year from the shipment date at a stable -20°C. However, the product is specifically designed for research use only and should not be used for clinical diagnosis or human use.
About Abbkine Scientific
Abbkine Scientific Company Limited is a life science research company headquartered in California. Founded in 2012, the establishment has been able to spread its tentacles across the globe with increasing presence and acceptance from Asia Pacific thanks to its continuous efforts to make the world a better place.
Abbkine combines cutting edge technology with manufacturing engineering and cost advantage to provide innovative, high-quality assay kits and other research and scientific products enhance life science fundamental research and drug discovery amongst others.
New Post has been published on Abbkine - Antibodies, proteins, biochemicals, assay kits for life science research
New Post has been published on http://www.abbkine.com/abbkine-announces-release-new-antibody-ipkine-hrp-goat-anti-mouse-igg-hcs/
Abbkine announces the release of its new antibody - IPKine™ HRP Goat Anti-Mouse IgG HCS
Wuhan, China. 430074. 26th April 2017. Abbkine Scientific is no doubt one of the world’s leading scientific research companies and the institution recently announced the addition of another antibody into the IPKine family. The IPKine™ HRP Goat Anti-Mouse IgG HCS is an HRP Secondary Antibody designed to help researchers and scientists get the best out of their works without necessarily breaking the bank.
The Anti-Mouse Heavy Chain comes in a liquid solution with such features as heavy chain/Fc fragment specific optimization, helping to eliminate light chain interference. Its absorption of serum proteins also helps to minimize cross-reactivity with immunoglobulins from other species, which are some of its unique features.
The antibody reacts with Fc portion of mouse IgG heavy chain, while it doesn’t react with the Fab portion of mouse IgG. It's cross-reaction with human, bovine and horse serum proteins has been specialized minimized, while it may cross-react with immunoglobulins from other species. It has no reactivity on non-immunoglobulin serum proteins.
With its host being the goat, the antibody’s applications include WB, IP and others, hence a co-IP. For optimal working conditions, the dilutions should be determined by the investigator through experiment. However, the suggested solutions are WB 1:10,000-1:100,000, IHC 1:500-1:5,000 and ELISA 1:5,000-1:100,000.
The IPKine™ HRP Goat Anti-Mouse IgG HCS can be stored for as long as one year from the shipment date at a stable -20°C. However, the product is specifically designed for research use only and should not be used for clinical diagnosis or human use.
About Abbkine Scientific
Abbkine Scientific Company Limited is a life science research company headquartered in California. Founded in 2012, the establishment has been able to spread its tentacles across the globe with increasing presence and acceptance from Asia Pacific thanks to its continuous efforts to make the world a better place.
Abbkine combines cutting edge technology with manufacturing engineering and cost advantage to provide innovative, high-quality assay kits and other research and scientific products enhance life science fundamental research and drug discovery amongst others.
New Post has been published on Abbkine - Antibodies, proteins, biochemicals, assay kits for life science research
Interests on http://www.abbkine.com/abbkine-announces-release-new-antibody-ipkine-hrp-goat-anti-mouse-igg-hcs/
Abbkine announces the release of its new antibody - IPKine™ HRP Goat Anti-Mouse IgG HCS
Wuhan, China. 430074. 26th April 2017. Abbkine Scientific is no doubt one of the world’s leading scientific research companies and the institution recently announced the addition of another antibody into the IPKine family. The IPKine™ HRP Goat Anti-Mouse IgG HCS is an HRP Secondary Antibod.... Click for moreAbbkine announces the release of its new antibody - IPKine™ HRP Goat Anti-Mouse IgG HCS.
