#cryo-electron microscopy

[intelligentliving]

Powerful Microscopes Enhanced With Algorithm That Sharpens Focus

A multi-institutional team of scientists has developed an “enhance” tool for powerful microscopes, improving their resolution and accuracy. These machines are crucial to revealing insights into medicine and biology – so when they improve, we all win. The team – including researchers from the New Mexico Consortium Los Alamos National...

[bpod-bpod]

Immune Avengers Assemble

There are membranes wrapped around all of our cells, and single-celled organisms like bacteria, too. But they do more than just hold everything in – tiny tunnel-like pores control the flow of important chemicals in and out. Usually this delicate balance helps life along. But here is a pore-making protein that can kill. The complement membrane attack complex (MAC) assembles from a group of proteins in our blood, triggered by our immune system to punch holes in nasty pathogens like bacteria – its long strands piercing the cell surface while precious chemicals burst out. Examining the structure of MAC using cryogenic electron microscopy allows scientists to spot a crucial step in how these avenging proteins come together – slotting together a series of identical protein pieces into a ring shape, before it can begin to penetrate the cell. This insight may help the design of drugs to help MAC tackle infections.

Written by John Ankers

Image generated from the RCSB Protein Data Bank (PDB), NGL Viewer – AS Rose et al. (2018) doi:10.1093/bioinformatics/bty419

Text summary about research from ARC Centre of Excellence in Advanced Molecular Imaging, Department of Biochemistry and Molecular Biology, Monash University, Victoria, Australia

Data files contained in the PDB archive are free of all copyright restrictions, with attribution

Research published in Nature Communications, August 2018

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[oupacademic]

The Nobel Prize in Chemistry 2017

The Nobel Prize in Chemistry 2017 was awarded to Jacques Dubochet, Joachim Frank, and Richard Henderson "for developing cryo-electron microscopy for the high-resolution structure determination of biomolecules in solution".

Oxford University Press is proud to publish Nobel Prize winners, including Joachim Frank, who authored Three-Dimensional Electron Microscopy of Macromolecular Assemblies:

“The problem posed by the basic incompatibility between the vacuum requirements and the naturally hydrated state of biological specimens has only recently been solved by cryo-electron microscopy (cryo-EM) techniques: the specimen is kept frozen, hydrated throughout the experiment, at a temperature (liquid nitrogen or helium) at which the vapor pressure of water is negligible.”

Congratulations to Jacques Dubochet, Joachim Frank, and Richard Henderson!

GIF created from “Structure of Alcohol Oxidase from Pichia pastoris by Cryo Electron Microscopy” video. CC BY 4.0 via Wikimedia Commons.

[bpod-bpod]

Frozen Herpes

Viruses are biological machines built to spread infection. They trick infected cells into replicating their genetic material, pumping it into shell-like containers called capsids ready to infect more cells. Different herpes viruses can cause everything from cold-sores and inherited disorders, to some forms of cancer, yet they share similar traits. This computer reconstruction of the herpes simplex virus is based on images of frozen virus particles captured using cryogenic electron microscopy. Shades of pink and purple highlight the virus’ 'portal', where genetic material pumps into the capsid – from the side (top) or looking up (below). Each pair of images is presented as a sort of 'magic eye' picture, or 3D stereogram. Understanding how the herpes machinery works at vital stages of infection may help to design new drugs that point the immune system in the right direction, combatting infections with this pretty pathogen that can last a lifetime.

Written by John Ankers

Image from work by Marion McElwee and colleagues

Medical Research Council, University of Glasgow Centre for Virus Research, Glasgow, UK

Image originally published under a Creative Commons Licence (BY 4.0)

Published in PLOS Biology, June 2018

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[bpod-bpod]

Attention to Detail

Since the very first microscope revealed a hidden world, countless gradual improvements have exposed ever-deeper layers. This procession of progress eventually led to the electron microscope, able to show the very atoms of materials. But the electron beam – used in place of light – destroyed biological material, limiting its insights to the inert world. Determined to see not just matter, but life, at the finest detail, Richard Henderson – born on this day in 1945 – managed to expose a protein from a plant cell’s membrane to a reduced electron beam and picture its structure according to how the electrons bounced off (left, from 1975). With 15 years more work and the advent of cryo-electron microscopy, which freezes samples to shield from the electrons, he determined the structure at the ultimate resolution. In the process he gave the world a new viewpoint on life, and earned a share of a 2017 Nobel Prize.

Written by Anthony Lewis

Portrait reproduced with permission from the MRC Laboratory of Molecular Biology, Cambridge, UK

Electron microscopy image published by R. Henderson & P. N. T. Unwin in Nature, September 1975

Copyright of the electron microscopy image held by Nature, reproduced with permission

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[neosciencehub]

New Magnetic Technology Revolutionizes Cryo-Electron Microscopy @neosciencehub #MagneticTechnology #CryoElectronMicroscopy #MagIC #neosciencehub

New Magnetic Technology Revolutionizes Cryo-Electron Microscopy

[markettrend24]

Global 2022-2028 Cryo-Electron Microscopy Industry Trends Survey and Prospects Report

Global 2022-2028 Cryo-Electron Microscopy Industry Trends Survey and Prospects Report

Global Cryo-Electron Microscopy Market- Size, Share and Market Forecasts 2028 This report focuses on the Global Cryo-Electron Microscopy Market trends, future forecasts, growth opportunities, key end-user industries, and market players. The objectives of the study are to present the key developments of the market across the globe. Based on the Cryo-Electron Microscopy market development status,…

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[ankushshirbhate720]

Analysis of cost structure analysis of Сrуо-Еlесtrоn Місrоѕсору Маrkеt & how to increase the growth of market for Сrуо-Еlесtrоn Місrоѕсору.

Basic principle of Сrуо-Еlесtrоn Місrоѕсору:

Сrуо-еlесtrоn mісrоѕсору rеfеrѕ tо аѕ Тrаnѕmіѕѕіоn Еlесtrоn Місrоѕсору (ТЕМѕ) thаt uѕе еlесtrоn bеаmѕ tо ехаmіnе аnd аnаlуzе ѕtruсturеѕ оf mаtеrіаlѕ аnd mоlесulеѕ аt аn аtоmіс ѕсаlе.

Сrуо-еlесtrоn mісrоѕсору іѕ uѕеd fоr undеrѕtаndіng thе funсtіоnѕ аnd іntеrасtіоnѕ оf bіоmоlесulеѕ аѕ іt іѕ fundаmеntаl tо thе bіосhеmіѕtrу іnduѕtrу. Тhіѕ ѕuрроrtѕ thе рrосеѕѕ undеrtаkеn tо dеvеlор іnnоvаtіvе аnd аdvаnсеd drugѕ аѕ wеll аѕ mеdісаl trеаtmеntѕ іn оrdеr tо trеаt ѕуmрtоmѕ оf dіѕеаѕеѕ.

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