Posting this analysis I did on a transfem Jake reading after crystal mentioned it in the discord because I'm proud of what I've written and I think there's some interesting stuff here.
The transmasc read of the Shirogorov twins is the one that is more obvious/easier of course but a transfem reading is very interesting and compelling. In this read Adonis's aggressive gendering of Naomi as his Wife would not be exclusively the enforcement of cisheteropatriarchal marriage, instead being an attempt to affirm her gender while ignoring the more complicated aspects of it and still both re-enforcing heteropatriarchal marriage and now also being a bit (a lot) of a chaser about it (or at least he is very transmisogynistic about their marriage). Jake identifying himself strongly and specifically as "a man" (instead of Naomi's less traditionally gendered identification as "a detective") reads as repression in this lens, as well as defining himself as a sort of Un-Naomi (an observation that valerie @ty-bayonet-betteridge had made) and if Naomi is the one with more gender going on then clearly they are the Trans One™ (and thus the Girl One™) leading to Jake, like Adonis, flattening Naomi's gender in order to validate his own (by taking ownership of the role he was forced into as the Self discarded by Naomi in order to be Naomi (Nikolai allowed Naomi to be a person and to transition but Jake was denied personhood and thus also transition.)) It is interesting that the things which most directly imply a transmasc read of the twins are introduced or implied by Jake himself, which implies that it either points to the more complicated gender stuff going on with both of them or is a symptom of the way Jake tries to "Own his masculinity." It also serves the purpose of further endearing KC to the twins' plight with Nikolai (and specifically Jake's suppression) by presenting it not only as abuse but transphobic abuse specifically. Which ties into Jack and how he is socially cis (regardless of which direction you read the twins as trans in) and has subdued his Naomi (and thus is also repressing the non-masculine aspects of his gender) which Jack specifically describes as him not being "broken" which through this lens reads as him repressing both his plurality and his transfemininity and speaks to an attitude towards both which Jake also has where they are both weaknesses which he should not have (another reason why he genders himself as male and antagonizes Naomi.)
I also think the Jake vs Jacob distinction is interesting because as far as I can remember Nikolai is the main person who calls him Jacob, although given that's how he is credited in the finale I could be forgetting something there (which if I am it is likely either Merlin calling him that or him "reclaiming" the name as part of becoming the detective prince/"manning up and getting a real job" both of which would back up my further analysis.) This brings to mind Bob and their name being the shortened form of a more masculine given name, which is explicitly a denial of gender identity by authority figures, which Nikolai and Merlin both are. Jake being credited as Jacob is thus a sign that he, like Jack has repressed/given up on his transfemininity in order to "grow up" and assume his role as the director of the FBI and the head of the Shirogorov clan.
It's definitely a very interesting read on the characters and one worthy of further analysis, although I would need to do a closer re-listen to do more.