How to filter semi-made steroid solution [email protected]
step 1:You'd better draw a small amount of air (about 1ml) into the syringe before filling with steroid solution. This air is used to purge the filter at the end ensuring the minimum sample is left in the filter.since the quanity is limited and worthwile
step 2:Load the steroid solution into the syringe
step 3:Attach the filter safely with a twisting motion, fix it firmly but do not over-tighten.
step 4:Hold the assembled syringe and filter vertically to wet the membrane evenly. This prevents air blocks and promotes high flow rates as the liquid is spread evenly over the membrane surface. The actual physical design of the syringe filter will have a big bearing on the possible flow-rate through the filter. step 5:Press the syringe plunger gently to push steroid through the filter. If possible, discard the first 0.25-0.5ml of sample because if there is any contamination present, it is likely to be higher in those first few microlitres of sample. If the back pressure ever increases significantly, change the filter as it may have plugged. Avoid pressing excessively as this could cause the filter housing to burst. step 6: Push the air through the filter to purge the housing and recover the maximum amount of steroid solution.