#fluorophore

[bpod-bpod]

Rhobo Meets Matrix

Close-up on the extracellular matrix (ECM) – the molecules and minerals that are outside cells but involved with their biochemical and mechanical signalling – made possible using a fluorophore called Rhobo6 that binds the sugar chain glycans abundant in ECM

Read the published research article here

Video from work by Antonio Fiore and colleagues

Janelia Research Campus, Howard Hughes Medical Institute (HHMI), Ashburn, VA, USA

Video contributed by the authors under a Creative Commons Attribution 4.0 International (CC BY 4.0) licence

Published in Nature Methods, February 2025

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[er-cryptid]

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[pauljacobevans]

Fluorescence Microscopy Meets DIY Fluid Management

Fluorescence microscopy is an optical technique that incorporates fluorescence or phosphorescence (as opposed to reflection and absorption) in order to study the properties of organic and inorganic substances. Not a stranger to bringing DIY techniques into the lab, [Philip] is using 3D printing resources to advance science and delight interns from labs everywhere. In fluorescence microscopy, a huge limiting factor that decreases the amount of data that can be gleaned from a single sample is the number of targets that can be labeled with fluorescent tags. However, overlap in the spectral emissions of fluorophores limits the fluorophores that can be …read more http://pje.fyi/PJb8lq

[biolegend]

Back by popular demand, the Fuzzy Fluors have returned to BioLegend with a new member in tow. APC/Fire™ 750 now rounds out the crew. Visit their webpage to learn more about each fluor and how you can get their puppet: www.biolegend.com/fluor_puppets

[biolegend]

BioLegend is proud to announce the release of its newest fluor: APC/Fire™ 750, which offers improved stability and minimal compensation requirements for multicolor flow cytometry. Visit the webpage to learn more and see how you can acquire your own Dragon Squeeze Toy.  www.biolegend.com/apc-fire750

[antisensescience]

What is fluorescence microscopy? And how is it used in the lab? Amy tells us more

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[kill3rtcell]

The LSRFortessa recognises APC-Cy7 and AlexaFluor700 in separate channels

The LSRFortessa recognises APC-Cy7 and AlexaFluor700 in separate channels

I think I love this machine about as much as it is legally possible for a man to love a machine.

[senses-working-overtime]

New York Times, September 14, 1914.

a remarkable new method of testing absolutely whether an apparently lead person is really dead and thus avoiding the possibility of premature burial...

Dr Icard's system depends on the question whether the blood is still in circulation or not, and consists of a subcutaneous injection of a small quantity of fluorescine, which is quite harmless, but one of the most violent coloring matters known.

If there be the slightest motion of the blood, the fluorescine, carried around the body, stains it a vivid golden yellow, while the eyes become a deep emerald green... Half an hour is stated to be enough to make the test.

The laity, while duly impressed by this neat method, are asking whether the persons who are alive and undergo the dyeing process, and who later recover, will lose the golden yellow tint and the green eyes, as Dr Icard describes them, "are transformed into superb emeralds, set like jewels in their sockets."

It may be added however, that fluorescine is one of the most transitory dyes known.

Fluorescein was first synthesized by Adolf von Baeyer in 1871.  Fluorescein is a fluorophore -- a component of a molecule which causes a molecule to be fluorescent.